Seroatlas · Human Serome Atlas

PUSL1

tRNA pseudouridine synthase-like 1

Also known as: FLJ90811, PUSL1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N0Z8
Gene: PUSL1
Ensembl: ENSG00000169972
Chromosome: 1
Canonical length: 303 aa
Protein class: Predicted intracellular proteins
Subcellular location: Vesicles

OverviewNCBI Gene

Predicted to enable pseudouridine synthase activity. Predicted to be involved in tRNA pseudouridine synthesis. Located in mitochondrion. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

303 residues, UniProt reviewed canonical sequence.

>Q8N0Z8|PUSL1
     1  MSSAPASGSV RARYLVYFQY VGTDFNGVAA VRGTQRAVGV QNYLEEAAER LNSVEPVRFT
    61  ISSRTDAGVH ALSNAAHLDV QRRSGRPPFP PEVLAEALNT HLRHPAIRVL RAFRVPSDFH
   121  ARHAATSRTY LYRLATGCHR RDELPVFERN LCWTLPADCL DMVAMQEAAQ HLLGTHDFSA
   181  FQSAGSPVPS PVRTLRRVSV SPGQASPLVT PEESRKLRFW NLEFESQSFL YRQVRRMTAV
   241  LVAVGLGALA PAQVKTILES QDPLGKHQTR VAPAHGLFLK SVLYGNLGAA SCTLQGPQFG
   301  SHG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PUSL1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 21 nTPM

Expression across tissuesHPA

Tissue

esophagus: 21 nTPM
skin: 18 nTPM
pancreas: 15 nTPM
pituitary gland: 15 nTPM
liver: 14 nTPM
adrenal gland: 14 nTPM

Single-cell type

esophageal apical cells: 276 nCPM
breast lactating cells: 56 nCPM
esophageal suprabasal cells: 53 nCPM
extravillous trophoblasts: 48 nCPM
esophageal basal cells: 46 nCPM
syncytiotrophoblasts: 42 nCPM

Immune cell

intermediate monocyte: 18 nTPM
non-classical monocyte: 16 nTPM
T-reg: 14 nTPM
classical monocyte: 13 nTPM
myeloid DC: 12 nTPM
memory CD4 T-cell: 11 nTPM

Brain region

choroid plexus: 13 nTPM
medulla oblongata: 12 nTPM
white matter: 11 nTPM
cerebral cortex: 10 nTPM
amygdala: 10 nTPM
pons: 9.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.98
gnomAD pLI: 0
gnomAD missense Z: -1.44
DepMap mean gene effect: -0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

tRNA pseudouridine synthesis

Molecular functions

Cellular components

mitochondrion

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PUSL1 as an antibody target. Whether an autoantibody or antibody against PUSL1 could matter depends on whether native PUSL1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PUSL1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PUSL1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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