Seroatlas · Human Serome Atlas

PRSS3

Trypsin-3

Also known as: PRSS4, TRY3, TRY3_HUMAN, TRY4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P35030
Gene
PRSS3
Ensembl
ENSG00000010438
Chromosome
9
Canonical length
304 aa
Protein class
Enzymes, Plasma proteins, Predicted intracellular proteins, Predicted secreted proteins
Subcellular location
Endoplasmic reticulum,Vesicles
Secretome location
Secreted to digestive system

OverviewNCBI Gene

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2010]

Canonical amino-acid sequenceUniProt

304 residues, UniProt reviewed canonical sequence.

>P35030|PRSS3
     1  MCGPDDRCPA RWPGPGRAVK CGKGLAAARP GRVERGGAQR GGAGLELHPL LGGRTWRAAR
    61  DADGCEALGT VAVPFDDDDK IVGGYTCEEN SLPYQVSLNS GSHFCGGSLI SEQWVVSAAH
   121  CYKTRIQVRL GEHNIKVLEG NEQFINAAKI IRHPKYNRDT LDNDIMLIKL SSPAVINARV
   181  STISLPTTPP AAGTECLISG WGNTLSFGAD YPDELKCLDA PVLTQAECKA SYPGKITNSM
   241  FCVGFLEGGK DSCQRDSGGP VVCNGQLQGV VSWGHGCAWK NRPGVYTKVY NYVDWIKDTI
   301  AANS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRSS3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
29,337 nTPM

Expression across tissuesHPA

Tissue

  • pancreas: 29,337 nTPM
  • esophagus: 124 nTPM
  • small intestine: 99 nTPM
  • duodenum: 87 nTPM
  • skin: 84 nTPM
  • colon: 82 nTPM

Single-cell type

  • pancreatic acinar cells: 9,292 nCPM
  • esophageal apical cells: 4,351 nCPM
  • enterocytes: 1,276 nCPM
  • colonocytes: 1,197 nCPM
  • gastric progenitor cells: 706 nCPM
  • tuft cells: 427 nCPM

Immune cell

  • basophil: 160 nTPM
  • intermediate monocyte: 1.5 nTPM
  • non-classical monocyte: 1.5 nTPM
  • MAIT T-cell: 1.2 nTPM
  • myeloid DC: 0.5 nTPM
  • memory CD8 T-cell: 0.4 nTPM

Brain region

  • cerebral cortex: 28 nTPM
  • hypothalamus: 20 nTPM
  • cerebellum: 20 nTPM
  • pons: 20 nTPM
  • hippocampal formation: 18 nTPM
  • white matter: 18 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.9
gnomAD pLI
0
gnomAD missense Z
-0.03
DepMap mean gene effect
-0.01
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRSS3 as an antibody target. Whether an autoantibody or antibody against PRSS3 could matter depends on whether native PRSS3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRSS3 is annotated as secreted, so native PRSS3 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label PRSS3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PRSS3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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