Seroatlas · Human Serome Atlas

PRSS21

Testisin

Also known as: ESP-1, TEST_HUMAN, TEST1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y6M0
Gene: PRSS21
Ensembl: ENSG00000007038
Chromosome: 16
Canonical length: 314 aa
Protein class: Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Cytosol,Mid piece,Principal piece
Secretome location: Intracellular and membrane

OverviewNCBI Gene

This gene encodes a cell-surface anchored serine protease, which is a member of the trypsin family of serine proteases. The encoded protein is predicted to be active on peptide linkages involving the carboxyl group of lysine or arginine. The encoded protein localizes to the cytoplasm and the plasma membrane of premeiotic testicular germ cells and may be involved in progression of testicular tumors of germ cell origin. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Canonical amino-acid sequenceUniProt

314 residues, UniProt reviewed canonical sequence.

>Q9Y6M0|PRSS21
     1  MGARGALLLA LLLARAGLRK PESQEAAPLS GPCGRRVITS RIVGGEDAEL GRWPWQGSLR
    61  LWDSHVCGVS LLSHRWALTA AHCFETYSDL SDPSGWMVQF GQLTSMPSFW SLQAYYTRYF
   121  VSNIYLSPRY LGNSPYDIAL VKLSAPVTYT KHIQPICLQA STFEFENRTD CWVTGWGYIK
   181  EDEALPSPHT LQEVQVAIIN NSMCNHLFLK YSFRKDIFGD MVCAGNAQGG KDACFGDSGG
   241  PLACNKNGLW YQIGVVSWGV GCGRPNRPGV YTNISHHFEW IQKLMAQSGM SQPDPSWPLL
   301  FFPLLWALPL LGPV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRSS21 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.32
Highest tissue expression: 82 nTPM

Expression across tissuesHPA

Tissue

testis: 82 nTPM
salivary gland: 20 nTPM
bone marrow: 7.4 nTPM
prostate: 4 nTPM
fallopian tube: 2.5 nTPM
lung: 2.5 nTPM

Single-cell type

early primary spermatocytes: 305 nCPM
late spermatids: 299 nCPM
differentiating spermatogonia: 137 nCPM
late primary spermatocytes: 94 nCPM
early spermatids: 59 nCPM
oocytes: 18 nCPM

Immune cell

eosinophil: 33 nTPM
basophil: 16 nTPM
MAIT T-cell: 4.8 nTPM
classical monocyte: 3.5 nTPM
gdT-cell: 3.2 nTPM
naive CD8 T-cell: 3.2 nTPM

Brain region

hypothalamus: 0.2 nTPM
medulla oblongata: 0.2 nTPM
amygdala: 0.1 nTPM
basal ganglia: 0.1 nTPM
cerebral cortex: 0.1 nTPM
pons: 0.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.32
gnomAD pLI: 0
gnomAD missense Z: -0.64
DepMap mean gene effect: -0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRSS21 as an antibody target. Whether an autoantibody or antibody against PRSS21 could matter depends on whether native PRSS21 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRSS21 is annotated at the cell surface, where native PRSS21 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label PRSS21 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...