PRODH2

Protein identityUniProt · HPA

UniProt accession

Q9UF12

Gene

PRODH2

Ensembl

ENSG00000250799

Chromosome

19

Canonical length

460 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Cytosol

OverviewNCBI Gene

The protein encoded by this gene catalyzes the first step in the catabolism of trans-4-hydroxy-L-proline, an amino acid derivative obtained through food intake and collagen turnover. One of the downstream products of this catabolism is glyoxylate, which in people with disorders of glyoxalate metabolism can lead to an increase in oxalate levels and the formation of calcium-oxalate kidney stones. Therefore, this gene may serve as a therapeutic target against primary hyperoxalurias (PH). This gene is similar to proline dehydrogenase (oxidase) 1, a mitochondrial enzyme that catalyzes the first step in proline catabolism. [provided by RefSeq, Jan 2017]

Canonical amino-acid sequenceUniProt

460 residues, UniProt reviewed canonical sequence.

>Q9UF12|PRODH2
     1  MLRTCYVLCS QAGPPSRGWQ SLSFDGGAFH LKGTGELTRA LLVLRLCAWP PLVTHGLLLQ
    61  AWSRRLLGSR LSGAFLRASV YGQFVAGETA EEVKGCVQQL RTLSLRPLLA VPTEEEPDSA
   121  AKSGEAWYEG NLGAMLRCVD LSRGLLEPPS LAEASLMQLK VTALTSTRLC KELASWVRRP
   181  GASLELSPER LAEAMDSGQN LQVSCLNAEQ NQHLRASLSR LHRVAQYARA QHVRLLVDAE
   241  YTSLNPALSL LVAALAVRWN SPGEGGPWVW NTYQACLKDT FERLGRDAEA AHRAGLAFGV
   301  KLVRGAYLDK ERAVAQLHGM EDPTQPDYEA TSQSYSRCLE LMLTHVARHG PMCHLMVASH
   361  NEESVRQATK RMWELGIPLD GTVCFGQLLG MCDHVSLALG QAGYVVYKSI PYGSLEEVIP
   421  YLIRRAQENR SVLQGARREQ ELLSQELWRR LLPGCRRIPH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRODH2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

188 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.19

gnomAD pLI

0

gnomAD missense Z

0.12

DepMap mean gene effect

-0.03

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• L-proline catabolic process
• L-proline catabolic process to L-glutamate

Molecular functions

• FAD binding
• proline dehydrogenase activity
• oxidoreductase activity, acting on the CH-NH group of donors

Cellular components

• mitochondrial inner membrane
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Proline dehydrogenase domain
• Proline oxidase family
• FAD-linked oxidoreductase-like
• Proline dehydrogenase

KeywordsUniProt

• Acetylation
• FAD
• Flavoprotein
• Oxidoreductase
• Proline metabolism