Seroatlas · Human Serome Atlas

PROB1

Proline-rich basic protein 1

Also known as: C5orf65, PROB1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: E7EW31
Gene: PROB1
Ensembl: ENSG00000228672
Chromosome: 5
Canonical length: 1015 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Located in nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1015 residues, UniProt reviewed canonical sequence.

>E7EW31|PROB1
     1  MLTALAPPAL PGIPRQLPTA PARRQDSSGS SGSYYTAPGS PEPPDVGPDA KGPANWPWVA
    61  PGRGAGAQPR LSVSAQNSRQ RHGPGSGFPR GPGSGPRPPQ PQLRTLPSGE MEVIFGVGPL
   121  FGCSGADDRE AQQQFTEPAF ISPLPPGPAS PAAVPRQSQV PDGGSRWATY LELRPRGPSP
   181  AAPAQFECVE VALEEGAAPA RPRTVPKRQI ELRPRPQSPP RAAGAPRPRL LLRTGSLDES
   241  LGPLQAAAGF VQTALARKLS PEAPAPSSAT FGSTGRSEPE TRETARSTHV VLEKAKSRPL
   301  RVRDNSAPAK APRPWPSLRE RAIRRDKPAP GTEPLGPVSS SIFLQSEEKI QEARKTRFPR
   361  EAPDRTVQRA RSPPFECRIP SEVPSRAVRP RSPSPPRQTP NGAVRGPRCP SPQNLSPWDR
   421  TTRRVSSPLF PEASSEWENQ NPAVEETVSR RSPSPPILSQ WNQCVAGERS PSLEAPSLWE
   481  IPHSAVADAV EPRSSPSPPA FFPWEAPDRP IGTWGPSPQE TWDPMGPGSS IAFTQEAQNG
   541  LTQEELAPPT PSAPGTPEPT EMQSPSTREI SDLAFGGSQQ SPEVAAPEPP GSHPVGTLDA
   601  DKCPEVLGPG EAASGRPRMA IPRPRDVRKL VKTTYAPGFP AGAQGSGLPA PPADPCGEEG
   661  GESKTQEPPA LGPPAPAHYT SVFIKDFLPV VPHPYEPPEP SFDTVARDAS QPNGVLRRRA
   721  ENSTAKPFKR TEIRLPGALA LGRRPEVTSR VRARGPGGEN RDVEAQRLVP DGDGRTSPLG
   781  GARSSSQRSP VGPAGVRSPR PGSPQMQASP SPGIAPKPKT PPTAPEPAAA VQAPLPREPL
   841  ALAGRTAPAQ PRAASAPPTD RSPQSPSQGA RRQPGAAPLG KVLVDPESGR YYFVEAPRQP
   901  RLRVLFDPES GQYVEVLLPP SSPGPPHRVY TPLALGLGLY PPAYGPIPSL SLPPSPGPQA
   961  LGSPQLPWVS EAGPLDGTYY LPVSGTPNPA PPLLLCAPPS SSGPTQPGKG SLFPL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PROB1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.7
Highest tissue expression: 76 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 76 nTPM
heart muscle: 25 nTPM
tongue: 17 nTPM
adrenal gland: 5.1 nTPM
pituitary gland: 3.1 nTPM
prostate: 3.1 nTPM

Single-cell type

thymic myoid cells: 27 nCPM
ependymal cells: 24 nCPM
myonuclei: 10 nCPM
epididymal efferent duct ciliated cells: 6.7 nCPM
cardiomyocytes: 6.5 nCPM
hepatic stellate cells: 6.5 nCPM

Immune cell

T-reg: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM

Brain region

midbrain: 16 nTPM
medulla oblongata: 13 nTPM
spinal cord: 12 nTPM
thalamus: 10 nTPM
hypothalamus: 8.8 nTPM
amygdala: 8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.71
gnomAD pLI: 0.01
gnomAD missense Z: 1.57
DepMap mean gene effect: -0.11
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Cellular components

nucleoplasm

Protein domainsUniProt · Pfam · InterPro

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PROB1 as an antibody target. Whether an autoantibody or antibody against PROB1 could matter depends on whether native PROB1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PROB1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PROB1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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