Seroatlas · Human Serome Atlas

PREP

Prolyl endopeptidase

Also known as: PPCE_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P48147
Gene
PREP
Ensembl
ENSG00000085377
Chromosome
6
Canonical length
710 aa
Protein class
Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins
Subcellular location
Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

710 residues, UniProt reviewed canonical sequence.

>P48147|PREP
     1  MLSLQYPDVY RDETAVQDYH GHKICDPYAW LEDPDSEQTK AFVEAQNKIT VPFLEQCPIR
    61  GLYKERMTEL YDYPKYSCHF KKGKRYFYFY NTGLQNQRVL YVQDSLEGEA RVFLDPNILS
   121  DDGTVALRGY AFSEDGEYFA YGLSASGSDW VTIKFMKVDG AKELPDVLER VKFSCMAWTH
   181  DGKGMFYNSY PQQDGKSDGT ETSTNLHQKL YYHVLGTDQS EDILCAEFPD EPKWMGGAEL
   241  SDDGRYVLLS IREGCDPVNR LWYCDLQQES SGIAGILKWV KLIDNFEGEY DYVTNEGTVF
   301  TFKTNRQSPN YRVINIDFRD PEESKWKVLV PEHEKDVLEW IACVRSNFLV LCYLHDVKNI
   361  LQLHDLTTGA LLKTFPLDVG SIVGYSGQKK DTEIFYQFTS FLSPGIIYHC DLTKEELEPR
   421  VFREVTVKGI DASDYQTVQI FYPSKDGTKI PMFIVHKKGI KLDGSHPAFL YGYGGFNISI
   481  TPNYSVSRLI FVRHMGGILA VANIRGGGEY GETWHKGGIL ANKQNCFDDF QCAAEYLIKE
   541  GYTSPKRLTI NGGSNGGLLV AACANQRPDL FGCVIAQVGV MDMLKFHKYT IGHAWTTDYG
   601  CSDSKQHFEW LVKYSPLHNV KLPEADDIQY PSMLLLTADH DDRVVPLHSL KFIATLQYIV
   661  GRSRKQSNPL LIHVDTKAGH GAGKPTAKVI EEVSDMFAFI ARCLNVDWIP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PREP can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.2
Highest tissue expression
61 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 61 nTPM
  • tongue: 44 nTPM
  • rectum: 35 nTPM
  • colon: 30 nTPM
  • skin: 25 nTPM
  • duodenum: 22 nTPM

Single-cell type

  • sertoli cells: 193 nCPM
  • myonuclei: 184 nCPM
  • cone photoreceptor cells: 184 nCPM
  • enteric stem cells: 162 nCPM
  • enteric transient amplifying cells: 136 nCPM
  • syncytiotrophoblasts: 130 nCPM

Immune cell

  • non-classical monocyte: 23 nTPM
  • NK-cell: 20 nTPM
  • memory CD8 T-cell: 17 nTPM
  • intermediate monocyte: 17 nTPM
  • MAIT T-cell: 17 nTPM
  • myeloid DC: 17 nTPM

Brain region

  • cerebral cortex: 25 nTPM
  • thalamus: 18 nTPM
  • white matter: 17 nTPM
  • choroid plexus: 16 nTPM
  • midbrain: 16 nTPM
  • hypothalamus: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.43
gnomAD pLI
0.08
gnomAD missense Z
2.27
DepMap mean gene effect
-0.02
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PREP as an antibody target. Whether an autoantibody or antibody against PREP could matter depends on whether native PREP is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PREP is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PREP as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PREP. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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