Seroatlas · Human Serome Atlas

PRAMEF20

PRAME family member 20

Also known as: OTTHUMT00000008157, PRA20_HUMAN, PRAMEF21

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5VT98
Gene: PRAMEF20
Ensembl: ENSG00000204478
Chromosome: 1
Canonical length: 475 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable ubiquitin-like ligase-substrate adaptor activity. Predicted to be involved in proteasome-mediated ubiquitin-dependent protein catabolic process. Predicted to be part of Cul2-RING ubiquitin ligase complex. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

475 residues, UniProt reviewed canonical sequence.

>Q5VT98|PRAMEF20
     1  MSIRTPPRLL ELAGRSLLRD EALAISTLEE LPTELFPPLF MEAFSRRHCE ALKLMVQAWP
    61  FLRLPLGSLM KRPCPETFQA VLDGLDALLT HRVRLRRWKL QVLDLQDVSE NFWMVWSEAM
   121  ARRCLPNAMM NRKPLQDCPR MRGQQPLTVF IDLCLKNRTL DEYFTCLFLW VKQREGLVHL
   181  CCKKLKMLGM LFHNIRNILK TVNLDCIQEV EVNCNWTLPV LAEFTPYLGQ MRNLRKLVLS
   241  DIDSRYISPE QKKEFVTQFT TQFLKLRCLQ KLYMNSVSFL EGHLDQMLSC LKTSLNILAI
   301  TNCVLLESDL KHLSKYPSIG QLKTLDLSGT RLANFSLVPL QVLLEKVAAT LEYLDLDDCG
   361  IVDSQVNAIL PALSRCFELT TFSFRGNPIS TATLENLLCH TIRLNNLCLE LYPAPRESYD
   421  VRGIVCRSRF AQLGAELMGR VRALREPERI LFCTDYCPQC GNRSLYDLEV DRCCC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRAMEF20 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.26
Highest tissue expression: 0 nTPM

Expression across tissuesHPA

Tissue

adipose tissue: 0 nTPM
adrenal gland: 0 nTPM
amygdala: 0 nTPM
appendix: 0 nTPM
basal ganglia: 0 nTPM
blood vessel: 0 nTPM

Single-cell type

myonuclei: 1.1 nCPM
differentiating spermatogonia: 0.2 nCPM
undifferentiated spermatogonia: 0.2 nCPM
brain inhibitory neurons: 0.1 nCPM
choroid plexus epithelial cells: 0.1 nCPM
fibro-adipogenic progenitors: 0.1 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

cerebellum: 0.1 nTPM
cerebral cortex: 0.1 nTPM
amygdala: 0 nTPM
basal ganglia: 0 nTPM
choroid plexus: 0 nTPM
hippocampal formation: 0 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.93
gnomAD pLI: 0
gnomAD missense Z: -0.52
DepMap mean gene effect: -0.28
DepMap dependency class: selective

OntologyGO

Biological processes

Molecular functions

ubiquitin-like ligase-substrate adaptor activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRAMEF20 as an antibody target. Whether an autoantibody or antibody against PRAMEF20 could matter depends on whether native PRAMEF20 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRAMEF20 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PRAMEF20 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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