Seroatlas · Human Serome Atlas

PRAMEF12

PRAME family member 12

Also known as: PRA12_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O95522
Gene: PRAMEF12
Ensembl: ENSG00000116726
Chromosome: 1
Canonical length: 483 aa
Protein class: Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Predicted to enable ubiquitin-like ligase-substrate adaptor activity. Predicted to be involved in proteasome-mediated ubiquitin-dependent protein catabolic process. Predicted to be part of Cul2-RING ubiquitin ligase complex. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

483 residues, UniProt reviewed canonical sequence.

>O95522|PRAMEF12
     1  MSLQAPPRLL ELAEQSLLRD RALAIPTLEE LPRELFPPLF MEAFTRRCCE TLTTMVQAWP
    61  FTCLPLGSLM KSCNLEIFRA VLEGLDALLA QKVRPRRWKL QVLDLRNVDE NFWGIWSGAS
   121  ALSPEALSKR RTAGNCPRPG GQQPLMVILD LCFKNGTLDE CLTHFLEWGK QRKGLLHVCC
   181  KELQIFGIAI HRIIEVLNTV ELDCIQEVEV CCPWELSILI RFAPYLGQMR NLRKLVLFNI
   241  HVSACIPLDR KEQFVIQFTS QFLKLDYFQK LYMHSVSFLE GHLDQLLRCL QAPLETVVMT
   301  ECLLSESDLK HLSWCPSIRQ LKELDLRGIT LTHFSPEPLS VLLEQAEATL QTLDLEDCGI
   361  VDSQLSAILP ALSRCSQLST FSFCGNLISM AALENLLRHT VGLSKLSLEL YPAPLESYDA
   421  QGALCWGRFS QLGAELMKTL RDLRQPKIIV FSTVPCPRCG IRASYDLEPS HCLLNACCQG
   481  GFI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRAMEF12 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.26
Highest tissue expression: 0.1 nTPM

Expression across tissuesHPA

Tissue

choroid plexus: 0.1 nTPM
adipose tissue: 0 nTPM
adrenal gland: 0 nTPM
amygdala: 0 nTPM
appendix: 0 nTPM
basal ganglia: 0 nTPM

Single-cell type

choroid plexus epithelial cells: 0.1 nCPM
ependymal cells: 0.1 nCPM
fallopian tube ciliated cells: 0.1 nCPM
adipocytes: 0 nCPM
adrenal cortex cells: 0 nCPM
adrenal medulla cells: 0 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

amygdala: 0 nTPM
basal ganglia: 0 nTPM
cerebellum: 0 nTPM
cerebral cortex: 0 nTPM
choroid plexus: 0 nTPM
hippocampal formation: 0 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.26
gnomAD pLI: 0
gnomAD missense Z: -1.13
DepMap mean gene effect: -0.08
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

ubiquitin-like ligase-substrate adaptor activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRAMEF12 as an antibody target. Whether an autoantibody or antibody against PRAMEF12 could matter depends on whether native PRAMEF12 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRAMEF12 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PRAMEF12 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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