Seroatlas · Human Serome Atlas

PRAMEF1

PRAME family member 1

Also known as: PRAM1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O95521
Gene: PRAMEF1
Ensembl: ENSG00000116721
Chromosome: 1
Canonical length: 474 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

This gene is a member of the PRAME (preferentially expressed antigen of melanoma) gene family which is expressed in many cancers but may function in reproductive tissues during development. Alternative promoter usage generates two transcript variants, which encode different isoforms. [provided by RefSeq, Jun 2014]

Canonical amino-acid sequenceUniProt

474 residues, UniProt reviewed canonical sequence.

>O95521|PRAMEF1
     1  MSIQAPPRLL ELAGQSLLRD QALSISAMEE LPRVLYLPLF MEAFSRRHFQ TLTVMVQAWP
    61  FTCLPLGSLM KTLHLETLKA LLEGLHMLLT QKDRPRRWKL QVLDLRDVDE NFWARWPGAW
   121  ALSCFPETTS KRQTAEDCPR MGEHQPLKVF IDICLKEIPQ DECLRYLFQW VYQRRGLVHL
   181  CCSKLVNYLT PIKYLRKSLK IIYLNSIQEL EIRNMSWPRL IRKLRCYLKE MKNLRKLVFS
   241  RCHHYTSDNE LEGRLVAKFS SVFLRLEHLQ LLKIKLITFF SGHLEQLIRC LQNPLENLEL
   301  TYGYLLEEDM KCLSQYPSLG YLKHLNLSYV LLFRISLEPL GALLEKIAAS LKTLILEGCQ
   361  IHYSQLSAIL PGLSRCSQLT TFYFGRNCMS IDALKDLLRH TSGLSKLSLE TYPAPEESLN
   421  SLVRVNWEIF TPLRAELMCT LREVRQPKRI FIGPTPCPSC GSSPSEELEL HLCC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PRAMEF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 0 nTPM

Expression across tissuesHPA

Tissue

adipose tissue: 0 nTPM
adrenal gland: 0 nTPM
amygdala: 0 nTPM
appendix: 0 nTPM
basal ganglia: 0 nTPM
blood vessel: 0 nTPM

Single-cell type

early spermatids: 8.6 nCPM
late primary spermatocytes: 5 nCPM
late spermatids: 2.9 nCPM
differentiating spermatogonia: 0.1 nCPM
adipocytes: 0 nCPM
adrenal cortex cells: 0 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

amygdala: 0 nTPM
basal ganglia: 0 nTPM
cerebellum: 0 nTPM
cerebral cortex: 0 nTPM
choroid plexus: 0 nTPM
hippocampal formation: 0 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.88
gnomAD pLI: 0
gnomAD missense Z: -2.13
DepMap mean gene effect: -0.03
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

ubiquitin-like ligase-substrate adaptor activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PRAMEF1 as an antibody target. Whether an autoantibody or antibody against PRAMEF1 could matter depends on whether native PRAMEF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PRAMEF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PRAMEF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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