Seroatlas · Human Serome Atlas

PPP1R12B

Protein phosphatase 1 regulatory subunit 12B

Also known as: MGC131980, MGC87886, MYPT2, MYPT2_HUMAN, PP1bp55

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O60237
Gene
PPP1R12B
Ensembl
ENSG00000077157
Chromosome
1
Canonical length
982 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Plasma membrane,Actin filaments

OverviewNCBI Gene

Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1, catalytic subunit delta), a large regulatory subunit (MYPT, myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2, the first of which is widely expressed, and the second of which may be specific to heart, skeletal muscle, and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome. [provided by RefSeq, Oct 2011]

Canonical amino-acid sequenceUniProt

982 residues, UniProt reviewed canonical sequence.

>O60237|PPP1R12B
     1  MAELEHLGGK RAESARMRRA EQLRRWRGSL TEQEPAERRG AGRQPLTRRG SPRVRFEDGA
    61  VFLAACSSGD TDEVRKLLAR GADINTVNVD GLTALHQACI DENLDMVKFL VENRANVNQQ
   121  DNEGWTPLHA AASCGYLNIA EYFINHGASV GIVNSEGEVP SDLAEEPAMK DLLLEQVKKQ
   181  GVDLEQSRKE EEQQMLQDAR QWLNSGKIED VRQARSGATA LHVAAAKGYS EVLRLLIQAG
   241  YELNVQDYDG WTPLHAAAHW GVKEACSILA EALCDMDIRN KLGQTPFDVA DEGLVEHLEL
   301  LQKKQNVLRS EKETRNKLIE SDLNSKIQSG FFKNKEKMLY EEETPKSQEM EEENKESSSS
   361  SSEEEEGEDE ASESETEKEA DKKPEAFVNH SNSESKSSIT EQIPAPAQNT FSASSARRFS
   421  SGLFNKPEEP KDESPSSWRL GLRKTGSHNM LSEVANSREP IRDRGSSIYR SSSSPRISAL
   481  LDNKDKEREN KSYISSLAPR KLNSTSDIEE KENRESAVNL VRSGSYTRQL WRDEAKGNEI
   541  PQTIAPSTYV STYLKRTPHK SQADTTAEKT ADNVSSSTPL CVITNRPLPS TANGVTATPV
   601  LSITGTDSSV EAREKRRSYL TPVRDEEAES LRKARSRQAR QTRRSTQGVT LTDLQEAERT
   661  FSRSRAERQA QEQPREKPTD TEGLEGSPEK HEPSAVPATE AGEGQQPWGR SLDEEPICHR
   721  LRCPAQPDKP TTPASPSTSR PSLYTSSHLL WTNRFSVPDS ESSETTTNTT TAKEMDKNEN
   781  EEADLDEQSS KRLSIRERRR PKERRRGTGI NFWTKDEDET DGSEEVKETW HERLSRLESG
   841  GSNPTTSDSY GDRASARARR EAREARLATL TSRVEEDSNR DYKKLYESAL TENQKLKTKL
   901  QEAQLELADI KSKLEKVAQQ KQEKTSDRSS VLEMEKRERR ALERKMSEME EEMKVLTELK
   961  SDNQRLKDEN GALIRVISKL SK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PPP1R12B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.55
Highest tissue expression
335 nTPM

Expression across tissuesHPA

Tissue

  • heart muscle: 335 nTPM
  • smooth muscle: 230 nTPM
  • endometrium: 208 nTPM
  • seminal vesicle: 187 nTPM
  • blood vessel: 149 nTPM
  • esophagus: 141 nTPM

Single-cell type

  • cardiomyocytes: 2,070 nCPM
  • myonuclei: 1,550 nCPM
  • ependymal cells: 986 nCPM
  • adipocytes: 757 nCPM
  • smooth muscle cells: 720 nCPM
  • thymic myoid cells: 630 nCPM

Immune cell

  • neutrophil: 16 nTPM
  • basophil: 2.2 nTPM
  • naive B-cell: 2.1 nTPM
  • memory B-cell: 2 nTPM
  • classical monocyte: 1.7 nTPM
  • plasmacytoid DC: 1.7 nTPM

Brain region

  • white matter: 93 nTPM
  • medulla oblongata: 77 nTPM
  • spinal cord: 75 nTPM
  • midbrain: 73 nTPM
  • choroid plexus: 73 nTPM
  • pons: 66 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.47
gnomAD pLI
0
gnomAD missense Z
0.97
DepMap mean gene effect
0
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PPP1R12B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PPP1R12B as an antibody target. Whether an autoantibody or antibody against PPP1R12B could matter depends on whether native PPP1R12B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PPP1R12B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PPP1R12B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PPP1R12B. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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