POLR2J2
DNA-directed RNA polymerase II subunit RPB11-b1
Also known as: RPB11b1, RPB1B_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q9GZM3
- Gene
- POLR2J2
- Ensembl
- ENSG00000228049
- Chromosome
- 7
- Canonical length
- 115 aa
- Protein class
- Predicted intracellular proteins, RNA polymerase related proteins
- Subcellular location
- Nucleoplasm
OverviewNCBI Gene
This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
115 residues, UniProt reviewed canonical sequence.
>Q9GZM3|POLR2J2
1 MNAPPAFESF LLFEGEKITI NKDTKVPKAC LFTINKEDHT LGNIIKSQLL KDPQVLFAGY
61 KVPHPLEHKI IIRVQTTPDY SPQEAFTNAI TDLISELSLL EERFRTCLLP LRLLPLocalizationUniProt · AlphaFold · HPA
Whether an antibody against POLR2J2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.38
- Highest tissue expression
- 10 nTPM
Expression across tissuesHPA
Tissue
- tonsil: 10 nTPM
- small intestine: 8.5 nTPM
- spleen: 5.8 nTPM
- skin: 5.4 nTPM
- stomach: 5.3 nTPM
- appendix: 4.8 nTPM
Single-cell type
- enterocytes: 0.1 nCPM
- adipocytes: 0 nCPM
- adrenal cortex cells: 0 nCPM
- adrenal medulla cells: 0 nCPM
- alveolar cells type 1: 0 nCPM
- alveolar cells type 2: 0 nCPM
Immune cell
- basophil: 15 nTPM
- naive B-cell: 10 nTPM
- memory B-cell: 9.9 nTPM
- naive CD4 T-cell: 9.2 nTPM
- plasmacytoid DC: 8.9 nTPM
- gdT-cell: 8.7 nTPM
Brain region
- cerebral cortex: 60 nTPM
- cerebellum: 40 nTPM
- thalamus: 39 nTPM
- white matter: 38 nTPM
- hypothalamus: 38 nTPM
- amygdala: 37 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.93
- gnomAD pLI
- 0.02
- gnomAD missense Z
- -0.23
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Molecular functions
Cellular components
- core complex
- RNA polymerase II
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads POLR2J2 as an antibody target. Whether an autoantibody or antibody against POLR2J2 could matter depends on whether native POLR2J2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
POLR2J2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label POLR2J2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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