Seroatlas · Human Serome Atlas

POLA2

DNA polymerase alpha subunit B

Also known as: DPOA2_HUMAN, FLJ21662

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q14181
Gene: POLA2
Ensembl: ENSG00000014138
Chromosome: 11
Canonical length: 598 aa
Protein class: Metabolic proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Basal body,Cytosol

OverviewNCBI Gene

Predicted to enable DNA binding activity. Involved in DNA replication initiation and DNA replication, synthesis of primer. Located in ciliary basal body; cytosol; and nucleoplasm. Part of alpha DNA polymerase:primase complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

598 residues, UniProt reviewed canonical sequence.

>Q14181|POLA2
     1  MSASAQQLAE ELQIFGLDCE EALIEKLVEL CVQYGQNEEG MVGELIAFCT STHKVGLTSE
    61  ILNSFEHEFL SKRLSKARHS TCKDSGHAGA RDIVSIQELI EVEEEEEILL NSYTTPSKGS
   121  QKRAISTPET PLTKRSVSTR SPHQLLSPSS FSPSATPSQK YNSRSNRGEV VTSFGLAQGV
   181  SWSGRGGAGN ISLKVLGCPE ALTGSYKSMF QKLPDIREVL TCKIEELGSE LKEHYKIEAF
   241  TPLLAPAQEP VTLLGQIGCD SNGKLNNKSV ILEGDREHSS GAQIPVDLSE LKEYSLFPGQ
   301  VVIMEGINTT GRKLVATKLY EGVPLPFYQP TEEDADFEQS MVLVACGPYT TSDSITYDPL
   361  LDLIAVINHD RPDVCILFGP FLDAKHEQVE NCLLTSPFED IFKQCLRTII EGTRSSGSHL
   421  VFVPSLRDVH HEPVYPQPPF SYSDLSREDK KQVQFVSEPC SLSINGVIFG LTSTDLLFHL
   481  GAEEISSSSG TSDRFSRILK HILTQRSYYP LYPPQEDMAI DYESFYVYAQ LPVTPDVLII
   541  PSELRYFVKD VLGCVCVNPG RLTKGQVGGT FARLYLRRPA ADGAERQSPC IAVQVVRI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLA2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.3
Highest tissue expression: 15 nTPM

Expression across tissuesHPA

Tissue

thymus: 15 nTPM
tonsil: 15 nTPM
lymph node: 13 nTPM
bone marrow: 10 nTPM
appendix: 6.7 nTPM
rectum: 6.4 nTPM

Single-cell type

podocytes: 45 nCPM
microglia: 23 nCPM
oligodendrocytes: 16 nCPM
papillary tip epithelial cells: 12 nCPM
renal collecting duct principal cells: 11 nCPM
choroid plexus epithelial cells: 10 nCPM

Immune cell

basophil: 11 nTPM
myeloid DC: 9.6 nTPM
plasmacytoid DC: 8.2 nTPM
T-reg: 5.8 nTPM
MAIT T-cell: 5.6 nTPM
total PBMC: 5.6 nTPM

Brain region

white matter: 5.8 nTPM
cerebellum: 5 nTPM
medulla oblongata: 4.5 nTPM
cerebral cortex: 4 nTPM
pons: 3.8 nTPM
midbrain: 3.4 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about POLA2.

Disease | GeneticClinVar

2 pathogenic / likely-pathogenic of 100 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Telomere Biology Disorder

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.65
gnomAD pLI: 0
gnomAD missense Z: 1.06
DepMap mean gene effect: -1.6
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

DNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

DNA polymerase alpha/delta/epsilon, subunit B
DNA polymerase alpha/epsilon subunit B
DNA polymerase alpha, subunit B, N-terminal
DNA polymerase alpha, subunit B
DNA polymerase alpha, subunit B, N-terminal domain superfamily
DNA polymerase alpha subunit B, OB domain
DNA polymerase alpha subunit B N-terminal
DNA polymerase alpha subunit B, OB domain

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLA2 as an antibody target. Whether an autoantibody or antibody against POLA2 could matter depends on whether native POLA2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLA2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLA2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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