Seroatlas · Human Serome Atlas

POFUT2

GDP-fucose protein O-fucosyltransferase 2

Also known as: C21orf80, FUT13, KIAA0958, OFUT2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9Y2G5
Gene
POFUT2
Ensembl
ENSG00000186866
Chromosome
21
Canonical length
429 aa
Protein class
Enzymes, Predicted intracellular proteins
Secretome location
Intracellular and membrane

OverviewNCBI Gene

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

429 residues, UniProt reviewed canonical sequence.

>Q9Y2G5|POFUT2
     1  MATLSFVFLL LGAVSWPPAS ASGQEFWPGQ SAADILSGAA SRRRYLLYDV NPPEGFNLRR
    61  DVYIRIASLL KTLLKTEEWV LVLPPWGRLY HWQSPDIHQV RIPWSEFFDL PSLNKNIPVI
   121  EYEQFIAESG GPFIDQVYVL QSYAEGWKEG TWEEKVDERP CIDQLLYSQD KHEYYRGWFW
   181  GYEETRGLNV SCLSVQGSAS IVAPLLLRNT SARSVMLDRA ENLLHDHYGG KEYWDTRRSM
   241  VFARHLREVG DEFRSRHLNS TDDADRIPFQ EDWMKMKVKL GSALGGPYLG VHLRRKDFIW
   301  GHRQDVPSLE GAVRKIRSLM KTHRLDKVFV ATDAVRKEYE ELKKLLPEMV RFEPTWEELE
   361  LYKDGGVAII DQWICAHARF FIGTSVSTFS FRIHEEREIL GLDPKTTYNR FCGDQEKACE
   421  QPTHWKITY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POFUT2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.27
Highest tissue expression
31 nTPM

Expression across tissuesHPA

Tissue

  • pituitary gland: 31 nTPM
  • ovary: 27 nTPM
  • choroid plexus: 24 nTPM
  • blood vessel: 21 nTPM
  • thyroid gland: 21 nTPM
  • adrenal gland: 20 nTPM

Single-cell type

  • late spermatids: 77 nCPM
  • late primary spermatocytes: 51 nCPM
  • endometrial ciliated cells: 43 nCPM
  • early spermatids: 39 nCPM
  • fallopian tube ciliated cells: 39 nCPM
  • respiratory ciliated cells: 34 nCPM

Immune cell

  • eosinophil: 0.8 nTPM
  • MAIT T-cell: 0.5 nTPM
  • neutrophil: 0.5 nTPM
  • naive CD4 T-cell: 0.4 nTPM
  • naive CD8 T-cell: 0.4 nTPM
  • plasmacytoid DC: 0.4 nTPM

Brain region

  • choroid plexus: 36 nTPM
  • hypothalamus: 20 nTPM
  • midbrain: 17 nTPM
  • medulla oblongata: 16 nTPM
  • pons: 16 nTPM
  • thalamus: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.74
gnomAD pLI
0
gnomAD missense Z
1.16
DepMap mean gene effect
-0.07
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POFUT2 as an antibody target. Whether an autoantibody or antibody against POFUT2 could matter depends on whether native POFUT2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POFUT2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POFUT2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/POFUT2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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