Seroatlas · Human Serome Atlas

PLEKHF1

Pleckstrin homology domain-containing family F member 1

Also known as: APPD, MGC4090, PHAFIN1, PKHF1_HUMAN, ZFYVE15

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96S99
Gene: PLEKHF1
Ensembl: ENSG00000166289
Chromosome: 19
Canonical length: 279 aa
Protein class: Predicted intracellular proteins, Transporters

OverviewNCBI Gene

Enables phosphatidylinositol-3-phosphate binding activity; phosphatidylinositol-4-phosphate binding activity; and phosphatidylinositol-5-phosphate binding activity. Involved in endosome organization; positive regulation of autophagy; and protein localization to plasma membrane. Located in endosome and lysosome. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

279 residues, UniProt reviewed canonical sequence.

>Q96S99|PLEKHF1
     1  MVDHLANTEI NSQRIAAVES CFGASGQPLA LPGRVLLGEG VLTKECRKKA KPRIFFLFND
    61  ILVYGSIVLN KRKYRSQHII PLEEVTLELL PETLQAKNRW MIKTAKKSFV VSAASATERQ
   121  EWISHIEECV RRQLRATGRP PSTEHAAPWI PDKATDICMR CTQTRFSALT RRHHCRKCGF
   181  VVCAECSRQR FLLPRLSPKP VRVCSLCYRE LAAQQRQEEA EEQGAGSPGQ PAHLARPICG
   241  ASSGDDDDSD EDKEGSRDGD WPSSVEFYAS GVAWSAFHS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PLEKHF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.33
Highest tissue expression: 88 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 88 nTPM
tongue: 36 nTPM
heart muscle: 29 nTPM
urinary bladder: 21 nTPM
adipose tissue: 20 nTPM
endometrium: 20 nTPM

Single-cell type

esophageal apical cells: 161 nCPM
cytotrophoblasts: 79 nCPM
peritubular myoid cells: 74 nCPM
leydig cells: 71 nCPM
nk-cells: 52 nCPM
decidual stromal cells: 50 nCPM

Immune cell

gdT-cell: 184 nTPM
memory CD8 T-cell: 147 nTPM
MAIT T-cell: 128 nTPM
naive CD8 T-cell: 83 nTPM
NK-cell: 83 nTPM
T-reg: 77 nTPM

Brain region

medulla oblongata: 24 nTPM
thalamus: 24 nTPM
white matter: 23 nTPM
midbrain: 20 nTPM
basal ganglia: 20 nTPM
pons: 17 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.16
gnomAD pLI: 0.01
gnomAD missense Z: 0.19
DepMap mean gene effect: -0.03
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PLEKHF1 as an antibody target. Whether an autoantibody or antibody against PLEKHF1 could matter depends on whether native PLEKHF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PLEKHF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PLEKHF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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