PLEKHA4

Protein identityUniProt · HPA

UniProt accession

Q9H4M7

Gene

PLEKHA4

Ensembl

ENSG00000105559

Chromosome

19

Canonical length

779 aa

Protein class

Predicted intracellular proteins

Subcellular location

Plasma membrane,Cytokinetic bridge,Centriolar satellite,Cytosol

OverviewNCBI Gene

This gene encodes a pleckstrin homology (PH) domain-containing protein. The PH domain is found near the N-terminus and contains a putative phosphatidylinositol 3, 4, 5-triphosphate-binding motif (PPBM). Elevated expression of this gene has been observed in some melanomas. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2017]

Canonical amino-acid sequenceUniProt

779 residues, UniProt reviewed canonical sequence.

>Q9H4M7|PLEKHA4
     1  MEGSRPRSSL SLASSASTIS SLSSLSPKKP TRAVNKIHAF GKRGNALRRD PNLPVHIRGW
    61  LHKQDSSGLR LWKRRWFVLS GHCLFYYKDS REESVLGSVL LPSYNIRPDG PGAPRGRRFT
   121  FTAEHPGMRT YVLAADTLED LRGWLRALGR ASRAEGDDYG QPRSPARPQP GEGPGGPGGP
   181  PEVSRGEEGR ISESPEVTRL SRGRGRPRLL TPSPTTDLHS GLQMRRARSP DLFTPLSRPP
   241  SPLSLPRPRS APARRPPAPS GDTAPPARPH TPLSRIDVRP PLDWGPQRQT LSRPPTPRRG
   301  PPSEAGGGKP PRSPQHWSQE PRTQAHSGSP TYLQLPPRPP GTRASMVLLP GPPLESTFHQ
   361  SLETDTLLTK LCGQDRLLRR LQEEIDQKQE EKEQLEAALE LTRQQLGQAT REAGAPGRAW
   421  GRQRLLQDRL VSVRATLCHL TQERERVWDT YSGLEQELGT LRETLEYLLH LGSPQDRVSA
   481  QQQLWMVEDT LAGLGGPQKP PPHTEPDSPS PVLQGEESSE RESLPESLEL SSPRSPETDW
   541  GRPPGGDKDL ASPHLGLGSP RVSRASSPEG RHLPSPQLGT KAPVARPRMS AQEQLERMRR
   601  NQECGRPFPR PTSPRLLTLG RTLSPARRQP DVEQRPVVGH SGAQKWLRSS GSWSSPRNTT
   661  PYLPTSEGHR ERVLSLSQAL ATEASQWHRM MTGGNLDSQG DPLPGVPLPP SDPTRQETPP
   721  PRSPPVANSG STGFSRRGSG RGGGPTPWGP AWDAGIAPPV LPQDEGAWPL RVTLLQSSF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PLEKHA4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.59

Highest tissue expression

34 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about PLEKHA4.

Disease | ImmuneIEDB

Conditions an epitope on PLEKHA4 was assayed in.

• glioblastoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.82

gnomAD pLI

0

gnomAD missense Z

0.61

DepMap mean gene effect

-0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• positive regulation of canonical Wnt signaling pathway
• positive regulation of Wnt signaling pathway, planar cell polarity pathway

Molecular functions

• identical protein binding
• phosphatidylinositol-3,4-bisphosphate binding
• phosphatidylinositol-3,5-bisphosphate binding
• phosphatidylinositol-3-phosphate binding
• phosphatidylinositol-4,5-bisphosphate binding

Cellular components

• cytoplasm
• extrinsic component of cytoplasmic side of plasma membrane
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Pleckstrin homology domain
• PH-like domain superfamily
• PKHA4-7, PH domain
• Pleckstrin homology domain-containing family A member 4-7, TBCA domain
• PH domain
• PH domain-containing proteins, TBCA domain

KeywordsUniProt

• Cytoplasm
• Lipid-binding
• Membrane
• Phosphoprotein