Seroatlas · Human Serome Atlas

PIGZ

GPI alpha-1,2-mannosyltransferase 4

Also known as: FLJ12768, MGC52163, PIGZ_HUMAN, SMP3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q86VD9
Gene: PIGZ
Ensembl: ENSG00000119227
Chromosome: 3
Canonical length: 579 aa
Protein class: Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

579 residues, UniProt reviewed canonical sequence.

>Q86VD9|PIGZ
     1  MQICGSSVAS VAAGTSFQVL GPVCWQQLDL KMAVRVLWGG LSLLRVLWCL LPQTGYVHPD
    61  EFFQSPEVMA EDILGVQAAR PWEFYPSSSC RSVLFPLLIS GSTFWLLRLW EELGPWPGLV
   121  SGYALLVGPR LLLTALSFAL DGAVYHLAPP MGADRWNALA LLSGSYVTLV FYTRTFSNTI
   181  EGLLFTWLLV LVSSHVTWGP TRKEPAPGPR WRSWLLGGIV AAGFFNRPTF LAFAVVPLYL
   241  WGTRGATNPG LKSLTREALV LLPGAALTAA VFVATDSWYF SSPATSRNLV LTPVNFLHYN
   301  LNPQNLARHG THARLTHLAV NGFLLFGVLH AQALQAAWQR LQVGLQASAQ MGLLRALGAR
   361  SLLSSPRSYL LLLYFMPLAL LSAFSHQEAR FLIPLLVPLV LLCSPQTQPV PWKGTVVLFN
   421  ALGALLFGCL HQGGLVPGLE YLEQVVHAPV LPSTPTHYTL LFTHTYMPPR HLLHLPGLGA
   481  PVEVVDMGGT EDWALCQTLK SFTRQPACQV AGGPWLCRLF VVTPGTTRRA VEKCSFPFKN
   541  ETLLFPHLTL EDPPALSSLL SGAWRDHLSL HIVELGEET

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PIGZ can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 8
Mean surface accessibility (rSASA): 0.27
Highest tissue expression: 37 nTPM

Expression across tissuesHPA

Tissue

colon: 37 nTPM
cerebellum: 25 nTPM
rectum: 16 nTPM
cerebral cortex: 13 nTPM
small intestine: 11 nTPM
basal ganglia: 10 nTPM

Single-cell type

colonocytes: 285 nCPM
goblet cells: 137 nCPM
prostatic hillock cells: 101 nCPM
enterocytes: 94 nCPM
enteric transient amplifying cells: 69 nCPM
enteric stem cells: 56 nCPM

Immune cell

intermediate monocyte: 0.8 nTPM
myeloid DC: 0.4 nTPM
classical monocyte: 0.3 nTPM
neutrophil: 0.3 nTPM
non-classical monocyte: 0.3 nTPM
eosinophil: 0.2 nTPM

Brain region

pons: 19 nTPM
cerebral cortex: 17 nTPM
hypothalamus: 17 nTPM
thalamus: 16 nTPM
midbrain: 16 nTPM
cerebellum: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.06
gnomAD pLI: 0
gnomAD missense Z: -0.04
DepMap mean gene effect: 0.05
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

GPI anchor biosynthetic process

Molecular functions

alpha-1,2-mannosyltransferase activity
mannosyltransferase activity
dol-P-Man:Man(3)GlcN-acyl-PI alpha-1,2-mannosyltransferase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PIGZ as an antibody target. Whether an autoantibody or antibody against PIGZ could matter depends on whether native PIGZ is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PIGZ is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PIGZ as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...