Seroatlas · Human Serome Atlas

PHLDA3

Pleckstrin homology-like domain family A member 3

Also known as: PHLA3_HUMAN, TIH1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y5J5
Gene: PHLDA3
Ensembl: ENSG00000174307
Chromosome: 1
Canonical length: 127 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Enables phosphatidylinositol phosphate binding activity and phosphatidylinositol-3,4-bisphosphate binding activity. Involved in intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; and positive regulation of apoptotic process. Located in plasma membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

127 residues, UniProt reviewed canonical sequence.

>Q9Y5J5|PHLDA3
     1  MTAAATATVL KEGVLEKRSG GLLQLWKRKR CVLTERGLQL FEAKGTGGRP KELSFARIKA
    61  VECVESTGRH IYFTLVTEGG GEIDFRCPLE DPGWNAQITL GLVKFKNQQA IQTVRARQSL
   121  GTGTLVS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PHLDA3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.38
Highest tissue expression: 145 nTPM

Expression across tissuesHPA

Tissue

adipose tissue: 145 nTPM
colon: 131 nTPM
breast: 114 nTPM
spinal cord: 91 nTPM
esophagus: 86 nTPM
urinary bladder: 74 nTPM

Single-cell type

late spermatids: 226 nCPM
esophageal apical cells: 184 nCPM
esophageal suprabasal cells: 179 nCPM
epididymal basal cells: 173 nCPM
ocular epithelial cells: 166 nCPM
schwann cells: 161 nCPM

Immune cell

neutrophil: 0.6 nTPM
T-reg: 0.6 nTPM
basophil: 0.3 nTPM
classical monocyte: 0.1 nTPM
gdT-cell: 0.1 nTPM
memory CD4 T-cell: 0.1 nTPM

Brain region

medulla oblongata: 143 nTPM
white matter: 127 nTPM
pons: 97 nTPM
thalamus: 97 nTPM
cerebellum: 96 nTPM
midbrain: 93 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.34
gnomAD pLI: 0.25
gnomAD missense Z: 0.15
DepMap mean gene effect: -0.01
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PHLDA3 as an antibody target. Whether an autoantibody or antibody against PHLDA3 could matter depends on whether native PHLDA3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PHLDA3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PHLDA3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...