Seroatlas · Human Serome Atlas

PHF21B

PHD finger protein 21B

Also known as: BHC80L, FLJ34161, PF21B_HUMAN, PHF4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96EK2
Gene: PHF21B
Ensembl: ENSG00000056487
Chromosome: 22
Canonical length: 531 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli fibrillar center,Cytosol

OverviewNCBI Gene

Predicted to enable zinc ion binding activity. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

531 residues, UniProt reviewed canonical sequence.

>Q96EK2|PHF21B
     1  MELQSRPEAL AVELARHQNG DLKKQLHERQ PRIAALSDKQ ALGTITAVPV TGPQVSSLQR
    61  LAGQGAAVLP QVRPKTLIPD SLPVAPGRDR PPKQPPTFQK ATVVSVKNPS PALPTANNTV
   121  SHVPAPGSQP QALAEPAALA SPLSSAGVAY AIISTSPSNA AAMAPSTAVS VVSDSIKVQP
   181  LLISADNKPP PRLLSSPHPA THHCPLHPSS LPLTPPSPSL SPSPLHGIFQ VIIIQPQVQT
   241  QPESTAESRP PTEEPSQGAQ ATKKKKEDRP PTQENPEKIA FMVALGLVTT EHLEEIQSKR
   301  QERKRRSTAN PAYSGLLETE RKRLASNYLN NPLFLTARAN EDPCWKNEIT HDEHCAACKR
   361  GANLQPCGTC PGAYHLSCLE PPLKTAPKGV WVCPRCQQKA LKKDEGVPWT GMLAIVHSYV
   421  THKTVKEEEK QKLLQRGSEL QNEHQQLEER DRRLASAVQK CLELKTSLLA RQRGTQSSLD
   481  RLRALLRLIQ GEQLLQVTMT TTSPAPLLAG PWTKPSVAAT HPTVQHPQGH N

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PHF21B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.62
Highest tissue expression: 18 nTPM

Expression across tissuesHPA

Tissue

pituitary gland: 18 nTPM
cervix: 8.4 nTPM
retina: 5.7 nTPM
basal ganglia: 5.2 nTPM
testis: 5.2 nTPM
amygdala: 5.1 nTPM

Single-cell type

somatotrophs: 351 nCPM
corticotrophs: 308 nCPM
bergmann glia: 307 nCPM
lactotrophs: 305 nCPM
podocytes: 266 nCPM
astrocytes: 230 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

medulla oblongata: 20 nTPM
midbrain: 20 nTPM
cerebral cortex: 19 nTPM
basal ganglia: 18 nTPM
thalamus: 16 nTPM
amygdala: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.29
gnomAD pLI: 0.99
gnomAD missense Z: 0.64
DepMap mean gene effect: -0.05
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

zinc ion binding

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PHF21B as an antibody target. Whether an autoantibody or antibody against PHF21B could matter depends on whether native PHF21B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PHF21B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PHF21B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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