Seroatlas · Human Serome Atlas

PHAF1

Phagosome assembly factor 1

Also known as: C16orf6, C16orf70, FLJ12076, lin-10, LIN10, PHAF1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9BSU1
Gene: PHAF1
Ensembl: ENSG00000125149
Chromosome: 16
Canonical length: 422 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoli fibrillar center,Nuclear speckles,Cytosol

OverviewNCBI Gene

Predicted to be involved in Golgi to plasma membrane protein transport. Located in phagophore assembly site. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

422 residues, UniProt reviewed canonical sequence.

>Q9BSU1|PHAF1
     1  MLDLEVVPER SLGNEQWEFT LGMPLAQAVA ILQKHCRIIK NVQVLYSEQS PLSHDLILNL
    61  TQDGIKLMFD AFNQRLKVIE VCDLTKVKLK YCGVHFNSQA IAPTIEQIDQ SFGATHPGVY
   121  NSAEQLFHLN FRGLSFSFQL DSWTEAPKYE PNFAHGLASL QIPHGATVKR MYIYSGNSLQ
   181  DTKAPMMPLS CFLGNVYAES VDVLRDGTGP AGLRLRLLAA GCGPGLLADA KMRVFERSVY
   241  FGDSCQDVLS MLGSPHKVFY KSEDKMKIHS PSPHKQVPSK CNDYFFNYFT LGVDILFDAN
   301  THKVKKFVLH TNYPGHYNFN IYHRCEFKIP LAIKKENADG QTETCTTYSK WDNIQELLGH
   361  PVEKPVVLHR SSSPNNTNPF GSTFCFGLQR MIFEVMQNNH IASVTLYGPP RPGSHLRTAE
   421  LP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PHAF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.3
Highest tissue expression: 54 nTPM

Expression across tissuesHPA

Tissue

liver: 54 nTPM
skin: 29 nTPM
skeletal muscle: 28 nTPM
spinal cord: 23 nTPM
bone marrow: 17 nTPM
esophagus: 17 nTPM

Single-cell type

endometrial glandular cells: 112 nCPM
hepatocytes: 100 nCPM
early primary spermatocytes: 64 nCPM
microglia: 63 nCPM
endometrial luminal cells: 55 nCPM
adrenal medulla cells: 53 nCPM

Immune cell

classical monocyte: 18 nTPM
myeloid DC: 15 nTPM
non-classical monocyte: 14 nTPM
intermediate monocyte: 12 nTPM
total PBMC: 8.9 nTPM
memory CD8 T-cell: 8.1 nTPM

Brain region

white matter: 32 nTPM
hippocampal formation: 27 nTPM
cerebral cortex: 25 nTPM
pons: 24 nTPM
spinal cord: 22 nTPM
basal ganglia: 22 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.31
gnomAD pLI: 0.98
DepMap mean gene effect: -0.01
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Golgi to plasma membrane protein transport

Cellular components

Protein domainsUniProt · Pfam · InterPro

PHAF1/Protein broad-minded
Phagosome assembly factor 1
Phagosome assembly factor 1

KeywordsUniProt

Cytoplasm

InteractionsUniProt · HPA

Protein binding partners of PHAF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PHAF1 as an antibody target. Whether an autoantibody or antibody against PHAF1 could matter depends on whether native PHAF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PHAF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PHAF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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