PFKL

Protein identityUniProt · HPA

UniProt accession

P17858

Gene

PFKL

Ensembl

ENSG00000141959

Chromosome

21

Canonical length

780 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoli,Mitochondria

OverviewNCBI Gene

This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Canonical amino-acid sequenceUniProt

780 residues, UniProt reviewed canonical sequence.

>P17858|PFKL
     1  MAAVDLEKLR ASGAGKAIGV LTSGGDAQGM NAAVRAVTRM GIYVGAKVFL IYEGYEGLVE
    61  GGENIKQANW LSVSNIIQLG GTIIGSARCK AFTTREGRRA AAYNLVQHGI TNLCVIGGDG
   121  SLTGANIFRS EWGSLLEELV AEGKISETTA RTYSHLNIAG LVGSIDNDFC GTDMTIGTDS
   181  ALHRIMEVID AITTTAQSHQ RTFVLEVMGR HCGYLALVSA LASGADWLFI PEAPPEDGWE
   241  NFMCERLGET RSRGSRLNII IIAEGAIDRN GKPISSSYVK DLVVQRLGFD TRVTVLGHVQ
   301  RGGTPSAFDR ILSSKMGMEA VMALLEATPD TPACVVTLSG NQSVRLPLME CVQMTKEVQK
   361  AMDDKRFDEA TQLRGGSFEN NWNIYKLLAH QKPPKEKSNF SLAILNVGAP AAGMNAAVRS
   421  AVRTGISHGH TVYVVHDGFE GLAKGQVQEV GWHDVAGWLG RGGSMLGTKR TLPKGQLESI
   481  VENIRIYGIH ALLVVGGFEA YEGVLQLVEA RGRYEELCIV MCVIPATISN NVPGTDFSLG
   541  SDTAVNAAME SCDRIKQSAS GTKRRVFIVE TMGGYCGYLA TVTGIAVGAD AAYVFEDPFN
   601  IHDLKVNVEH MTEKMKTDIQ RGLVLRNEKC HDYYTTEFLY NLYSSEGKGV FDCRTNVLGH
   661  LQQGGAPTPF DRNYGTKLGV KAMLWLSEKL REVYRKGRVF ANAPDSACVI GLKKKAVAFS
   721  PVTELKKDTD FEHRMPREQW WLSLRLMLKM LAQYRISMAA YVSGELEHVT RRTLSMDKGF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PFKL can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.2

Highest tissue expression

94 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.03

gnomAD pLI

0

gnomAD missense Z

1.68

DepMap mean gene effect

0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• canonical glycolysis
• fructose 1,6-bisphosphate metabolic process
• fructose 6-phosphate metabolic process
• glycolytic process
• negative regulation of insulin secretion
• response to glucose

Molecular functions

• 6-phosphofructokinase activity
• ATP binding
• fructose binding
• fructose-6-phosphate binding
• identical protein binding
• kinase binding
• metal ion binding

Cellular components

• 6-phosphofructokinase complex
• cytosol
• extracellular exosome
• extracellular region
• ficolin-1-rich granule lumen
• membrane
• secretory granule lumen

Protein domainsUniProt · Pfam · InterPro

• Phosphofructokinase domain
• ATP-dependent 6-phosphofructokinase, eukaryotic-type
• Phosphofructokinase, conserved site
• ATP-dependent 6-phosphofructokinase
• Phosphofructokinase superfamily
• ATP-dependent 6-phosphofructokinase, vertebrate-type
• Phosphofructokinase

KeywordsUniProt

• Acetylation
• Allosteric enzyme
• ATP-binding
• Cytoplasm
• Glycolysis
• Glycoprotein
• Kinase
• Magnesium
• Metal-binding
• Nucleotide-binding
• Phosphoprotein
• Transferase

InteractionsUniProt · HPA

Protein binding partners of PFKL in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PFKM