Seroatlas · Human Serome Atlas

PEMT

Phosphatidylethanolamine N-methyltransferase

Also known as: PEMPT, PEMT_HUMAN, PEMT2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9UBM1
Gene
PEMT
Ensembl
ENSG00000133027
Chromosome
17
Canonical length
199 aa
Protein class
Enzymes, Metabolic proteins, Predicted membrane proteins
Subcellular location
Endoplasmic reticulum,Vesicles,Mitochondria,Cytosol

OverviewNCBI Gene

Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]

Canonical amino-acid sequenceUniProt

199 residues, UniProt reviewed canonical sequence.

>Q9UBM1|PEMT
     1  MTRLLGYVDP LDPSFVAAVI TITFNPLYWN VVARWEHKTR KLSRAFGSPY LACYSLSVTI
    61  LLLNFLRSHC FTQAMLSQPR MESLDTPAAY SLGLALLGLG VVLVLSSFFA LGFAGTFLGD
   121  YFGILKEARV TVFPFNILDN PMYWGSTANY LGWAIMHASP TGLLLTVLVA LTYIVALLYE
   181  EPFTAEIYRQ KASGSHKRS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PEMT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
3
Mean surface accessibility (rSASA)
0.29
Highest tissue expression
259 nTPM

Expression across tissuesHPA

Tissue

  • liver: 259 nTPM
  • epididymis: 157 nTPM
  • breast: 91 nTPM
  • adipose tissue: 67 nTPM
  • cervix: 42 nTPM
  • testis: 40 nTPM

Single-cell type

  • epididymal principal cells: 958 nCPM
  • decidual stromal cells: 440 nCPM
  • epididymal efferent duct absorptive cells: 376 nCPM
  • peritubular myoid cells: 361 nCPM
  • hepatocytes: 272 nCPM
  • pancreatic islet cells: 232 nCPM

Immune cell

  • classical monocyte: 41 nTPM
  • intermediate monocyte: 39 nTPM
  • myeloid DC: 31 nTPM
  • NK-cell: 29 nTPM
  • naive B-cell: 28 nTPM
  • non-classical monocyte: 28 nTPM

Brain region

  • pons: 24 nTPM
  • white matter: 24 nTPM
  • cerebellum: 22 nTPM
  • midbrain: 21 nTPM
  • hypothalamus: 20 nTPM
  • spinal cord: 20 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.67
gnomAD pLI
0.23
gnomAD missense Z
-0.11
DepMap mean gene effect
-0.07
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

  • phosphatidyl-N-methylethanolamine N-methyltransferase activity
  • phosphatidylethanolamine N-methyltransferase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Phospholipid methyltransferase
  • Phosphatidyl-N-methylethanolamine/Phosphatidylethanolamine N-methyltransferase
  • Phospholipid methyltransferase

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PEMT as an antibody target. Whether an autoantibody or antibody against PEMT could matter depends on whether native PEMT is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PEMT is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PEMT as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PEMT. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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