PCIF1

Protein identityUniProt · HPA

UniProt accession

Q9H4Z3

Gene

PCIF1

Ensembl

ENSG00000100982

Chromosome

20

Canonical length

704 aa

Protein class

Enzymes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Microtubules,Cytokinetic bridge

OverviewNCBI Gene

Enables RNA polymerase II C-terminal domain phosphoserine binding activity; S-adenosyl-L-methionine binding activity; and mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity. Involved in mRNA processing; negative regulation of translation; and positive regulation of translation. Located in intercellular bridge; microtubule cytoskeleton; and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

704 residues, UniProt reviewed canonical sequence.

>Q9H4Z3|PCIF1
     1  MANENHGSPR EEASLLSHSP GTSNQSQPCS PKPIRLVQDL PEELVHAGWE KCWSRRENRP
    61  YYFNRFTNQS LWEMPVLGQH DVISDPLGLN ATPLPQDSSL VETPPAENKP RKRQLSEEQP
   121  SGNGVKKPKI EIPVTPTGQS VPSSPSIPGT PTLKMWGTSP EDKQQAALLR PTEVYWDLDI
   181  QTNAVIKHRG PSEVLPPHPE VELLRSQLIL KLRQHYRELC QQREGIEPPR ESFNRWMLER
   241  KVVDKGSDPL LPSNCEPVVS PSMFREIMND IPIRLSRIKF REEAKRLLFK YAEAARRLIE
   301  SRSASPDSRK VVKWNVEDTF SWLRKDHSAS KEDYMDRLEH LRRQCGPHVS AAAKDSVEGI
   361  CSKIYHISLE YVKRIREKHL AILKENNISE EVEAPEVEPR LVYCYPVRLA VSAPPMPSVE
   421  MHMENNVVCI RYKGEMVKVS RNYFSKLWLL YRYSCIDDSA FERFLPRVWC LLRRYQMMFG
   481  VGLYEGTGLQ GSLPVHVFEA LHRLFGVSFE CFASPLNCYF RQYCSAFPDT DGYFGSRGPC
   541  LDFAPLSGSF EANPPFCEEL MDAMVSHFER LLESSPEPLS FIVFIPEWRE PPTPALTRME
   601  QSRFKRHQLI LPAFEHEYRS GSQHICKKEE MHYKAVHNTA VLFLQNDPGF AKWAPTPERL
   661  QELSAAYRQS GRSHSSGSSS SSSSEAKDRD SGREQGPSRE PHPT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PCIF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.32

Highest tissue expression

43 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.16

gnomAD pLI

1

gnomAD missense Z

2.41

DepMap mean gene effect

-0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 13% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• methylation
• mRNA processing
• negative regulation of translation
• positive regulation of translation

Molecular functions

• RNA polymerase II C-terminal domain binding
• RNA polymerase II C-terminal domain phosphoserine binding
• S-adenosyl-L-methionine binding
• mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity

Cellular components

• intercellular bridge
• microtubule cytoskeleton
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• WW domain
• Heat shock protein 70kD, C-terminal domain superfamily
• WW domain superfamily
• WW domain
• PCIF1, WW domain
• mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase PCIF1-like
• Phosphorylated CTD interacting factor 1 WW domain

KeywordsUniProt

• Methyltransferase
• Nucleus
• Phosphoprotein
• Transferase

InteractionsUniProt · HPA

Protein binding partners of PCIF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• POLR2A