P4HA3

Protein identityUniProt · HPA

UniProt accession

Q7Z4N8

Gene

P4HA3

Ensembl

ENSG00000149380

Chromosome

11

Canonical length

544 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Cytosol

Secretome location

Intracellular and membrane

OverviewNCBI Gene

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Canonical amino-acid sequenceUniProt

544 residues, UniProt reviewed canonical sequence.

>Q7Z4N8|P4HA3
     1  MGPGARLAAL LAVLALGTGD PERAAARGDT FSALTSVARA LAPERRLLGL LRRYLRGEEA
    61  RLRDLTRFYD KVLSLHEDST TPVANPLLAF TLIKRLQSDW RNVVHSLEAS ENIRALKDGY
   121  EKVEQDLPAF EDLEGAARAL MRLQDVYMLN VKGLARGVFQ RVTGSAITDL YSPKRLFSLT
   181  GDDCFQVGKV AYDMGDYYHA IPWLEEAVSL FRGSYGEWKT EDEASLEDAL DHLAFAYFRA
   241  GNVSCALSLS REFLLYSPDN KRMARNVLKY ERLLAESPNH VVAEAVIQRP NIPHLQTRDT
   301  YEGLCQTLGS QPTLYQIPSL YCSYETNSNA YLLLQPIRKE VIHLEPYIAL YHDFVSDSEA
   361  QKIRELAEPW LQRSVVASGE KQLQVEYRIS KSAWLKDTVD PKLVTLNHRI AALTGLDVRP
   421  PYAEYLQVVN YGIGGHYEPH FDHATSPSSP LYRMKSGNRV ATFMIYLSSV EAGGATAFIY
   481  ANLSVPVVRN AALFWWNLHR SGEGDSDTLH AGCPVLVGDK WVANKWIHEY GQEFRRPCSS
   541  SPED

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against P4HA3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.3

Highest tissue expression

9.7 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.94

gnomAD pLI

0

gnomAD missense Z

0.53

DepMap mean gene effect

-0.06

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• collagen fibril organization

Molecular functions

• iron ion binding
• L-ascorbic acid binding
• procollagen-proline 4-dioxygenase activity

Cellular components

• endoplasmic reticulum
• endoplasmic reticulum lumen

Protein domainsUniProt · Pfam · InterPro

• Oxoglutarate/iron-dependent dioxygenase domain
• Prolyl 4-hydroxylase, alpha subunit
• Tetratricopeptide-like helical domain superfamily
• Prolyl 4-hydroxylase, N-terminal
• Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain
• Prolyl 4-hydroxylase
• Prolyl 4-hydroxylase, peptide-substrate-binding domain
• Prolyl 4-Hydroxylase alpha-subunit, N-terminal region
• 2OG-Fe(II) oxygenase superfamily
• Prolyl 4-hydroxylase peptide-substrate-binding domain

KeywordsUniProt

• Coiled coil
• Dioxygenase
• Endoplasmic reticulum
• Glycoprotein
• Iron
• Metal-binding
• Oxidoreductase
• Signal
• TPR repeat
• Vitamin C