P3H3

Protein identityUniProt · HPA

UniProt accession

Q8IVL6

Gene

P3H3

Ensembl

ENSG00000110811

Chromosome

12

Canonical length

736 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Nucleoli,Plasma membrane,Cytosol

Secretome location

Intracellular and membrane

OverviewNCBI Gene

The protein encoded by this gene belongs to the leprecan family of proteoglycans, which function as collagen prolyl hydroxylases that are required for proper collagen biosynthesis, folding and assembly. This protein, like other family members, is thought to reside in the endoplasmic reticulum. Epigenetic inactivation of this gene is associated with breast and other cancers, suggesting that it may function as a tumor suppressor. [provided by RefSeq, Aug 2013]

Canonical amino-acid sequenceUniProt

736 residues, UniProt reviewed canonical sequence.

>Q8IVL6|P3H3
     1  MLRLLRPLLL LLLLPPPGSP EPPGLTQLSP GAPPQAPDLL YADGLRAYAA GAWAPAVALL
    61  REALRSQAAL GRVRLDCGAS CAADPGAALP AVLLGAPEPD SGPGPTQGSW ERQLLRAALR
   121  RADCLTQCAA RRLGPGGAAR LRVGSALRDA FRRREPYNYL QRAYYQLKKL DLAAAAAHTF
   181  FVANPMHLQM REDMAKYRRM SGVRPQSFRD LETPPHWAAY DTGLELLGRQ EAGLALPRLE
   241  EALQGSLAQM ESCRADCEGP EEQQGAEEEE DGAASQGGLY EAIAGHWIQV LQCRQRCVGE
   301  TATRPGRSFP VPDFLPNQLR RLHEAHAQVG NLSQAIENVL SVLLFYPEDE AAKRALNQYQ
   361  AQLGEPRPGL GPREDIQRFI LRSLGEKRQL YYAMEHLGTS FKDPDPWTPA ALIPEALREK
   421  LREDQEKRPW DHEPVKPKPL TYWKDVLLLE GVTLTQDSRQ LNGSERAVLD GLLTPAECGV
   481  LLQLAKDAAG AGARSGYRGR RSPHTPHERF EGLTVLKAAQ LARAGTVGSQ GAKLLLEVSE
   541  RVRTLTQAYF SPERPLHLSF THLVCRSAIE GEQEQRMDLS HPVHADNCVL DPDTGECWRE
   601  PPAYTYRDYS GLLYLNDDFQ GGDLFFTEPN ALTVTARVRP RCGRLVAFSS GVENPHGVWA
   661  VTRGRRCALA LWHTWAPEHR EQEWIEAKEL LQESQEEEEE EEEEMPSKDP SPEPPSRRHQ
   721  RVQDKTGRAP RVREEL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against P3H3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.32

Highest tissue expression

15 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.01

gnomAD pLI

0

DepMap mean gene effect

-0.14

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• collagen biosynthetic process
• collagen metabolic process
• negative regulation of cell population proliferation
• peptidyl-lysine hydroxylation

Molecular functions

• iron ion binding
• L-ascorbic acid binding
• procollagen-proline 3-dioxygenase activity

Cellular components

• catalytic complex
• endoplasmic reticulum

Protein domainsUniProt · Pfam · InterPro

• Oxoglutarate/iron-dependent dioxygenase domain
• Prolyl 4-hydroxylase, alpha subunit
• Tetratricopeptide-like helical domain superfamily
• Prolyl 3-hydroxylase
• Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain
• Leprecan-like alpha-helical domain
• 2OG-Fe(II) oxygenase superfamily
• Leprecan family TPR-like repeat region

KeywordsUniProt

• Coiled coil
• Dioxygenase
• Endoplasmic reticulum
• Glycoprotein
• Iron
• Metal-binding
• Oxidoreductase
• Repeat
• Signal
• TPR repeat
• Vitamin C

InteractionsUniProt · HPA

Protein binding partners of P3H3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• P3H4