Seroatlas · Human Serome Atlas

OPLAH

5-oxoprolinase

Also known as: 5-Opase, OPLA, OPLA_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O14841
Gene: OPLAH
Ensembl: ENSG00000178814
Chromosome: 8
Canonical length: 1288 aa
Protein class: Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Potential drug targets, Predicted intracellular proteins
Subcellular location: Nucleoli fibrillar center
Quaternary structure: Homodimer

OverviewNCBI Gene

The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD). [provided by RefSeq, Jun 2012]

Canonical amino-acid sequenceUniProt

1288 residues, UniProt reviewed canonical sequence.

>O14841|OPLAH
     1  MGSPEGRFHF AIDRGGTFTD VFAQCPGGHV RVLKLLSEDP ANYADAPTEG IRRILEQEAG
    61  MLLPRDQPLD SSHIASIRMG TTVATNALLE RKGERVALLV TRGFRDLLHI GTQARGDLFD
   121  LAVPMPEVLY EEVLEVDERV VLHRGEAGTG TPVKGRTGDL LEVQQPVDLG ALRGKLEGLL
   181  SRGIRSLAVV LMHSYTWAQH EQQVGVLARE LGFTHVSLSS EAMPMVRIVP RGHTACADAY
   241  LTPAIQRYVQ GFCRGFQGQL KDVQVLFMRS DGGLAPMDTF SGSSAVLSGP AGGVVGYSAT
   301  TYQQEGGQPV IGFDMGGTST DVSRYAGEFE HVFEASTAGV TLQAPQLDIN TVAAGGGSRL
   361  FFRSGLFVVG PESAGAHPGP ACYRKGGPVT VTDANLVLGR LLPASFPCIF GPGENQPLSP
   421  EASRKALEAV ATEVNSFLTN GPCPASPLSL EEVAMGFVRV ANEAMCRPIR ALTQARGHDP
   481  SAHVLACFGG AGGQHACAIA RALGMDTVHI HRHSGLLSAL GLALADVVHE AQEPCSLLYA
   541  PETFVQLDQR LSRLEEQCVD ALQAQGFPRS QISTESFLHL RYQGTDCALM VSAHQHPATA
   601  RSPRAGDFGA AFVERYMREF GFVIPERPVV VDDVRVRGTG RSGLRLEDAP KAQTGPPRVD
   661  KMTQCYFEGG YQETPVYLLA ELGYGHKLHG PCLIIDSNST ILVEPGCQAE VTKTGDICIS
   721  VGAEVPGTVG PQLDPIQLSI FSHRFMSIAE QMGRILQRTA ISTNIKERLD FSCALFGPDG
   781  GLVSNAPHIP VHLGAMQETV QFQIQHLGAD LHPGDVLLSN HPSAGGSHLP DLTVITPVFW
   841  PGQTRPVFYV ASRGHHADIG GITPGSMPPH STMLQQEGAV FLSFKLVQGG VFQEEAVTEA
   901  LRAPGKVPNC SGTRNLHDNL SDLRAQVAAN QKGIQLVGEL IGQYGLDVVQ AYMGHIQANA
   961  ELAVRDMLRA FGTSRQARGL PLEVSSEDHM DDGSPIRLRV QISLSQGSAV FDFSGTGPEV
  1021  FGNLNAPRAV TLSALIYCLR CLVGRDIPLN QGCLAPVRVV IPRGSILDPS PEAAVVGGNV
  1081  LTSQRVVDVI LGAFGACAAS QGCMNNVTLG NAHMGYYETV AGGAGAGPSW HGRSGVHSHM
  1141  TNTRITDPEI LESRYPVILR RFELRRGSGG RGRFRGGDGV TRELLFREEA LLSVLTERRA
  1201  FRPYGLHGGE PGARGLNLLI RKNGRTVNLG GKTSVTVYPG DVFCLHTPGG GGYGDPEDPA
  1261  PPPGSPPQAL AFPEHGSVYE YRRAQEAV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against OPLAH can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.2
Highest tissue expression: 63 nTPM

Expression across tissuesHPA

Tissue

testis: 63 nTPM
heart muscle: 33 nTPM
liver: 33 nTPM
skeletal muscle: 33 nTPM
stomach: 19 nTPM
kidney: 18 nTPM

Single-cell type

late spermatids: 400 nCPM
early spermatids: 134 nCPM
late primary spermatocytes: 56 nCPM
cardiomyocytes: 47 nCPM
retinal pigment epithelial cells: 34 nCPM
neutrophils: 34 nCPM

Immune cell

classical monocyte: 1.1 nTPM
neutrophil: 1.1 nTPM
intermediate monocyte: 0.3 nTPM
total PBMC: 0.3 nTPM
myeloid DC: 0.1 nTPM
non-classical monocyte: 0.1 nTPM

Brain region

pons: 34 nTPM
medulla oblongata: 26 nTPM
cerebellum: 25 nTPM
white matter: 21 nTPM
choroid plexus: 19 nTPM
midbrain: 19 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about OPLAH.

Disease | AllUniProt

Conditions OPLAH is implicated in, by any mechanism.

5-oxoprolinase deficiency (OPLAHD) MIM:260005

Disease | GeneticClinVar

46 pathogenic / likely-pathogenic of 641 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

5-Oxoprolinase deficiency
OPLAH-related disorder

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.87
gnomAD pLI: 0
gnomAD missense Z: 0.27

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 11% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

glutathione metabolic process

Molecular functions

ATP binding
identical protein binding
5-oxoprolinase (ATP-hydrolyzing) activity

Cellular components

cytosol

Protein domainsUniProt · Pfam · InterPro

Hydantoinase A/oxoprolinase
Hydantoinase B/oxoprolinase
Hydantoinase/oxoprolinase, N-terminal
Oxoprolinase-like
Acetophenone carboxylase-like, C-terminal domain
Hydantoinase/oxoprolinase
Hydantoinase B/oxoprolinase
Hydantoinase/oxoprolinase N-terminal region
Hydantoinase/oxoprolinase C-terminal domain

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads OPLAH as an antibody target. Whether an autoantibody or antibody against OPLAH could matter depends on whether native OPLAH is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

OPLAH is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label OPLAH as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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