ONECUT3

Protein identityUniProt · HPA

UniProt accession

O60422

Gene

ONECUT3

Ensembl

ENSG00000205922

Chromosome

19

Canonical length

494 aa

Protein class

Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm

OverviewNCBI Gene

Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be located in chromatin. Predicted to be part of transcription regulator complex. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

494 residues, UniProt reviewed canonical sequence.

>O60422|ONECUT3
     1  MELSLESLGG LHSVAHAQAG ELLSPGHARS AAAQHRGLVA PGRPGLVAGM ASLLDGGGGG
    61  GGGGAGGAGG AGSAGGGADF RGELAGPLHP AMGMACEAPG LGGTYTTLTP LQHLPPLAAV
   121  ADKFHQHAAA AAVAGAHGGH PHAHPHPAAA PPPPPPPQRL AASVSGSFTL MRDERAALAS
   181  VGHLYGPYGK ELPAMGSPLS PLPNALPPAL HGAPQPPPPP PPPPLAAYGP PGHLAGDKLL
   241  PPAAFEPHAA LLGRAEDALA RGLPGGGGGT GSGGAGSGSA AGLLAPLGGL AAAGAHGPHG
   301  GGGGPGGSGG GPSAGAAAEE INTKEVAQRI TAELKRYSIP QAIFAQRILC RSQGTLSDLL
   361  RNPKPWSKLK SGRETFRRMW KWLQEPEFQR MSALRLAACK RKEQEQQKER ALQPKKQRLV
   421  FTDLQRRTLI AIFKENKRPS KEMQVTISQQ LGLELNTVSN FFMNARRRCM NRWAEEPSTA
   481  PGGPAGATAT FSKA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ONECUT3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.64

Highest tissue expression

4.1 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.69

gnomAD pLI

0

gnomAD missense Z

1.3

DepMap mean gene effect

0.02

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 0% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• regulation of transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription activator activity, RNA polymerase II-specific
• DNA-binding transcription factor activity, RNA polymerase II-specific
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• sequence-specific double-stranded DNA binding

Cellular components

• chromatin
• nucleus
• transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

• Homeodomain
• CUT domain
• Homedomain-like superfamily
• Lambda repressor-like, DNA-binding domain superfamily
• CUT Homeobox Domain-Containing Protein
• Homeodomain
• CUT domain

KeywordsUniProt

• Activator
• DNA-binding
• Homeobox
• Nucleus
• Transcription
• Transcription regulation