Seroatlas · Human Serome Atlas

NTHL1

Endonuclease III-like protein 1

Also known as: NTH_HUMAN, NTH1, OCTS3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P78549
Gene
NTHL1
Ensembl
ENSG00000065057
Chromosome
16
Canonical length
304 aa
Protein class
Cancer-related genes, Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins

OverviewNCBI Gene

The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity. [provided by RefSeq, Oct 2008]

Canonical amino-acid sequenceUniProt

304 residues, UniProt reviewed canonical sequence.

>P78549|NTHL1
     1  MTALSARMLT RSRSLGPGAG PRGCREEPGP LRRREAAAEA RKSHSPVKRP RKAQRLRVAY
    61  EGSDSEKGEG AEPLKVPVWE PQDWQQQLVN IRAMRNKKDA PVDHLGTEHC YDSSAPPKVR
   121  RYQVLLSLML SSQTKDQVTA GAMQRLRARG LTVDSILQTD DATLGKLIYP VGFWRSKVKY
   181  IKQTSAILQQ HYGGDIPASV AELVALPGVG PKMAHLAMAV AWGTVSGIAV DTHVHRIANR
   241  LRWTKKATKS PEETRAALEE WLPRELWHEI NGLLVGFGQQ TCLPVHPRCH ACLNQALCPA
   301  AQGL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NTHL1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.39
Highest tissue expression
25 nTPM

Expression across tissuesHPA

Tissue

  • basal ganglia: 25 nTPM
  • liver: 24 nTPM
  • cerebral cortex: 21 nTPM
  • cerebellum: 21 nTPM
  • midbrain: 18 nTPM
  • amygdala: 18 nTPM

Single-cell type

  • hepatocytes: 1,087 nCPM
  • respiratory secretory cells: 81 nCPM
  • goblet cells: 71 nCPM
  • enteric stem cells: 64 nCPM
  • conjunctival goblet cells: 50 nCPM
  • megakaryocytes: 50 nCPM

Immune cell

  • memory B-cell: 38 nTPM
  • T-reg: 34 nTPM
  • naive B-cell: 33 nTPM
  • plasmacytoid DC: 26 nTPM
  • intermediate monocyte: 26 nTPM
  • myeloid DC: 26 nTPM

Brain region

  • pons: 14 nTPM
  • medulla oblongata: 14 nTPM
  • thalamus: 13 nTPM
  • cerebral cortex: 13 nTPM
  • cerebellum: 12 nTPM
  • basal ganglia: 12 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NTHL1.

Disease | AllUniProt

Conditions NTHL1 is implicated in, by any mechanism.

  • Familial adenomatous polyposis 3 (FAP3) MIM:616415

Disease | GeneticClinVar

153 pathogenic / likely-pathogenic of 1,779 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.6
gnomAD pLI
0
gnomAD missense Z
-0.8
DepMap mean gene effect
-0.02
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NTHL1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NTHL1 as an antibody target. Whether an autoantibody or antibody against NTHL1 could matter depends on whether native NTHL1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NTHL1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NTHL1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NTHL1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...