Seroatlas · Human Serome Atlas

NT5DC4

5'-nucleotidase domain-containing protein 4

Also known as: NT5D4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q86YG4
Gene
NT5DC4
Ensembl
ENSG00000144130
Chromosome
2
Canonical length
428 aa
Protein class
Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable 5'-nucleotidase activity. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

428 residues, UniProt reviewed canonical sequence.

>Q86YG4|NT5DC4
     1  MPAWIFVNRS LALGKIRCFG FDMDYTLAAY KSPAYEALTF ELLLERLVCI GYPHEILRYT
    61  YDPTFPTRRL VFDELYGNLL KVDAHGNVLL GAYGFTFLSE AEIWSFYPSK FIQRDDLQCF
   121  YILNMLFNLP ETYLYACLVD FFSGCSRYTN CDTGYQHGNL FMSFRSLFQD VTDAMNNIHQ
   181  SGCLKKTLED LEKYVKKDPR LPILLGKMKE VGKVFLATNS SYNYTNAIMT YLFSISEAEA
   241  SGRPWRSYFD LIVVDTQKPH FFAEGLVLRQ VNTDSGKLHV GTYTGPHQHC AVYSGGSSDM
   301  VCELLGVRGM DILYIGDHIF GDILKSKKRQ GWRTCLVVPE LSWELDIWAQ EKERLEELKR
   361  LDTHLADIYQ HMDGSSCELQ VINFTKREIQ MPHESVVEQE QANLDPASCL LSCNQRSLPA
   421  KSCLSSAI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NT5DC4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Unknown
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.31
Highest tissue expression
2.4 nTPM

Expression across tissuesHPA

Tissue

  • testis: 2.4 nTPM
  • bone marrow: 1.1 nTPM
  • lymph node: 0.8 nTPM
  • tonsil: 0.5 nTPM
  • spleen: 0.3 nTPM
  • appendix: 0.2 nTPM

Single-cell type

  • early spermatids: 36 nCPM
  • endometrial luminal cells: 12 nCPM
  • late spermatids: 9.1 nCPM
  • sertoli cells: 5.6 nCPM
  • endometrial glandular cells: 4.6 nCPM
  • urothelial cells: 3.4 nCPM

Immune cell

  • eosinophil: 0.9 nTPM
  • neutrophil: 0.2 nTPM
  • intermediate monocyte: 0.1 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • gdT-cell: 0 nTPM

Brain region

  • cerebral cortex: 0.2 nTPM
  • thalamus: 0.2 nTPM
  • hippocampal formation: 0.1 nTPM
  • medulla oblongata: 0.1 nTPM
  • midbrain: 0.1 nTPM
  • pons: 0.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.93
gnomAD pLI
0
gnomAD missense Z
0.62

OntologyGO

Biological processes

Molecular functions

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NT5DC4 as an antibody target. Whether an autoantibody or antibody against NT5DC4 could matter depends on whether native NT5DC4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NT5DC4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NT5DC4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NT5DC4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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