Seroatlas · Human Serome Atlas

NT5C3B

7-methylguanosine phosphate-specific 5'-nucleotidase

Also known as: 5NT3B_HUMAN, cN-IIIB, MGC20781, NT5C3L

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q969T7
Gene: NT5C3B
Ensembl: ENSG00000141698
Chromosome: 17
Canonical length: 300 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable 5'-nucleotidase activity. Predicted to be involved in nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay. Predicted to be located in cytosol. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

300 residues, UniProt reviewed canonical sequence.

>Q969T7|NT5C3B
     1  MAEEVSTLMK ATVLMRQPGR VQEIVGALRK GGGDRLQVIS DFDMTLSRFA YNGKRCPSSY
    61  NILDNSKIIS EECRKELTAL LHHYYPIEID PHRTVKEKLP HMVEWWTKAH NLLCQQKIQK
   121  FQIAQVVRES NAMLREGYKT FFNTLYHNNI PLFIFSAGIG DILEEIIRQM KVFHPNIHIV
   181  SNYMDFNEDG FLQGFKGQLI HTYNKNSSAC ENSGYFQQLE GKTNVILLGD SIGDLTMADG
   241  VPGVQNILKI GFLNDKVEER RERYMDSYDI VLEKDETLDV VNGLLQHILC QGVQLEMQGP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NT5C3B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.26
Highest tissue expression: 11 nTPM

Expression across tissuesHPA

Tissue

pancreas: 11 nTPM
basal ganglia: 9.9 nTPM
choroid plexus: 9.4 nTPM
cerebral cortex: 8.7 nTPM
amygdala: 8.6 nTPM
cerebellum: 8.4 nTPM

Single-cell type

late primary spermatocytes: 203 nCPM
late spermatids: 163 nCPM
retinal horizontal cells: 100 nCPM
early primary spermatocytes: 85 nCPM
early spermatids: 76 nCPM
esophageal basal cells: 70 nCPM

Immune cell

MAIT T-cell: 2.5 nTPM
myeloid DC: 2.4 nTPM
naive B-cell: 2.4 nTPM
classical monocyte: 2.3 nTPM
memory CD4 T-cell: 2.3 nTPM
naive CD8 T-cell: 2 nTPM

Brain region

cerebellum: 18 nTPM
cerebral cortex: 14 nTPM
basal ganglia: 13 nTPM
hippocampal formation: 13 nTPM
hypothalamus: 13 nTPM
white matter: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.46
gnomAD pLI: 0
gnomAD missense Z: 0.43
DepMap mean gene effect: -0.19
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NT5C3B as an antibody target. Whether an autoantibody or antibody against NT5C3B could matter depends on whether native NT5C3B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NT5C3B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NT5C3B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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