Seroatlas · Human Serome Atlas

NT5C1B

Cytosolic 5'-nucleotidase 1B

Also known as: 5NT1B_HUMAN, AIRP, CN-IB

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96P26
Gene
NT5C1B
Ensembl
ENSG00000185013
Chromosome
2
Canonical length
610 aa
Protein class
Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nucleoli,Plasma membrane,Connecting piece,Mid piece

OverviewNCBI Gene

Cytosolic 5-prime nucleotidases, such as NT5C1B, catalyze production of adenosine, which regulates diverse physiologic processes (Sala-Newby and Newby, 2001 [PubMed 11690631]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

610 residues, UniProt reviewed canonical sequence.

>Q96P26|NT5C1B
     1  MSQTSLKQKK NEPGMRSSKE SLEAEKRKES DKTGVRLSNQ MRRAVNPNHS LRCCPFQGHS
    61  SCRRCLCAAE GTALGPCHTI RIYIHMCLLW EQGQQITMMR GSQESSLRKT DSRGYLVRSQ
   121  WSRISRSPST KAPSIDEPRS RNTSAKLPSS STSSRTPSTS PSLHDSSPPP LSGQPSLQPP
   181  ASPQLPRSLD SRPPTPPEPD PGSRRSTKMQ ENPEAWAQGI VREIRQTRDS QPLEYSRTSP
   241  TEWKSSSQRR GIYPASTQLD RNSLSEQQQQ QREDEDDYEA AYWASMRSFY EKNPSCSRPW
   301  PPKPKNAITI ALSSCALFNM VDGRKIYEQE GLEKYMEYQL TNENVILTPG PAFRFVKALQ
   361  YVNARLRDLY PDEQDLFDIV LMTNNHAQVG VRLINSVNHY GLLIDRFCLT GGKDPIGYLK
   421  AYLTNLYIAA DSEKVQEAIQ EGIASATMFD GAKDMAYCDT QLRVAFDGDA VLFSDESEHF
   481  TKEHGLDKFF QYDTLCESKP LAQGPLKGFL EDLGRLQKKF YAKNERLLCP IRTYLVTARS
   541  AASSGARVLK TLRRWGLEID EALFLAGAPK SPILVKIRPH IFFDDHMFHI EGAQRLGSIA
   601  AYGFNKKFSS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NT5C1B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.45
Highest tissue expression
34 nTPM

Expression across tissuesHPA

Tissue

  • testis: 34 nTPM
  • skin: 1 nTPM
  • esophagus: 0.9 nTPM
  • stomach: 0.7 nTPM
  • duodenum: 0.6 nTPM
  • gallbladder: 0.6 nTPM

Single-cell type

  • late spermatids: 15 nCPM
  • early spermatids: 4.2 nCPM
  • late primary spermatocytes: 1.8 nCPM
  • cardiomyocytes: 0.3 nCPM
  • peritubular myoid cells: 0.3 nCPM
  • epididymal basal cells: 0.2 nCPM

Immune cell

  • plasmacytoid DC: 1.2 nTPM
  • naive CD4 T-cell: 0.8 nTPM
  • MAIT T-cell: 0.7 nTPM
  • naive CD8 T-cell: 0.7 nTPM
  • gdT-cell: 0.4 nTPM
  • memory CD4 T-cell: 0.4 nTPM

Brain region

  • choroid plexus: 6.4 nTPM
  • midbrain: 2 nTPM
  • thalamus: 1.8 nTPM
  • medulla oblongata: 1.7 nTPM
  • basal ganglia: 1.6 nTPM
  • pons: 1.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.98
gnomAD pLI
0
gnomAD missense Z
0.24
DepMap mean gene effect
0.09
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NT5C1B as an antibody target. Whether an autoantibody or antibody against NT5C1B could matter depends on whether native NT5C1B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NT5C1B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NT5C1B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NT5C1B. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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