NT5C

Protein identityUniProt · HPA

UniProt accession

Q8TCD5

Gene

NT5C

Ensembl

ENSG00000125458

Chromosome

17

Canonical length

201 aa

Protein class

Metabolic proteins, Predicted intracellular proteins

Subcellular location

Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ribonucleotides, but not 5' ribonucleotides. Of the different forms of nucleotidases characterized, this enzyme is unique in its preference for 5'-dNTP. It may be one of the enzymes involved in regulating the size of dNTP pools in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011]

Canonical amino-acid sequenceUniProt

201 residues, UniProt reviewed canonical sequence.

>Q8TCD5|NT5C
     1  MARSVRVLVD MDGVLADFEA GLLRGFRRRF PEEPHVPLEQ RRGFLAREQY RALRPDLADK
    61  VASVYEAPGF FLDLEPIPGA LDAVREMNDL PDTQVFICTS PLLKYHHCVG EKYRWVEQHL
   121  GPQFVERIIL TRDKTVVLGD LLIDDKDTVR GQEETPSWEH ILFTCCHNRH LVLPPTRRRL
   181  LSWSDNWREI LDSKRGAAQR E

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NT5C can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.27

Highest tissue expression

47 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.25

gnomAD pLI

0

gnomAD missense Z

-0.25

DepMap mean gene effect

-0.13

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• allantoin metabolic process
• amide catabolic process
• dCMP catabolic process
• dephosphorylation
• dGMP catabolic process
• dTMP catabolic process
• dUMP catabolic process
• IMP catabolic process
• pyrimidine deoxyribonucleotide catabolic process
• UMP catabolic process

Molecular functions

• 5'-nucleotidase activity
• identical protein binding
• metal ion binding
• nucleotidase activity
• pyrimidine nucleotide binding

Cellular components

• cytoplasm
• cytosol
• extracellular exosome
• mitochondrion
• nucleus

Protein domainsUniProt · Pfam · InterPro

• 5'(3')-deoxyribonucleotidase
• HAD superfamily
• HAD-like superfamily
• 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C)

KeywordsUniProt

• Cytoplasm
• Hydrolase
• Magnesium
• Metal-binding
• Nucleotide metabolism
• Nucleotide-binding
• Phosphoprotein