Seroatlas · Human Serome Atlas

NPFFR2

Neuropeptide FF receptor 2

Also known as: GPR74, NPFF2, NPFF2_HUMAN, NPGPR

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9Y5X5
Gene
NPFFR2
Ensembl
ENSG00000056291
Chromosome
4
Canonical length
522 aa
Protein class
G-protein coupled receptors, Predicted membrane proteins
Subcellular location
Plasma membrane,Actin filaments

OverviewNCBI Gene

This gene encodes a member of a subfamily of G-protein-coupled neuropeptide receptors. This protein is activated by the neuropeptides A-18-amide (NPAF) and F-8-amide (NPFF) and may function in pain modulation and regulation of the opioid system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]

Canonical amino-acid sequenceUniProt

522 residues, UniProt reviewed canonical sequence.

>Q9Y5X5|NPFFR2
     1  MNSFFGTPAA SWCLLESDVS SAPDKEAGRE RRALSVQQRG GPAWSGSLEW SRQSAGDRRR
    61  LGLSRQTAKS SWSRSRDRTC CCRRAWWILV PAADRARRER FIMNEKWDTN SSENWHPIWN
   121  VNDTKHHLYS DINITYVNYY LHQPQVAAIF IISYFLIFFL CMMGNTVVCF IVMRNKHMHT
   181  VTNLFILNLA ISDLLVGIFC MPITLLDNII AGWPFGNTMC KISGLVQGIS VAASVFTLVA
   241  IAVDRFQCVV YPFKPKLTIK TAFVIIMIIW VLAITIMSPS AVMLHVQEEK YYRVRLNSQN
   301  KTSPVYWCRE DWPNQEMRKI YTTVLFANIY LAPLSLIVIM YGRIGISLFR AAVPHTGRKN
   361  QEQWHVVSRK KQKIIKMLLI VALLFILSWL PLWTLMMLSD YADLSPNELQ IINIYIYPFA
   421  HWLAFGNSSV NPIIYGFFNE NFRRGFQEAF QLQLCQKRAK PMEAYALKAK SHVLINTSNQ
   481  LVQESTFQNP HGETLLYRKS AEKPQQELVM EELKETTNSS EI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NPFFR2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
7
Mean surface accessibility (rSASA)
0.45
Highest tissue expression
6.7 nTPM

Expression across tissuesHPA

Tissue

  • thymus: 6.7 nTPM
  • seminal vesicle: 3 nTPM
  • placenta: 1.5 nTPM
  • epididymis: 0.8 nTPM
  • retina: 0.7 nTPM
  • amygdala: 0.4 nTPM

Single-cell type

  • breast lactating cells: 380 nCPM
  • early spermatids: 40 nCPM
  • extravillous trophoblasts: 29 nCPM
  • retinal horizontal cells: 28 nCPM
  • other brain neurons: 28 nCPM
  • syncytiotrophoblasts: 21 nCPM

Immune cell

  • basophil: 0.3 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • hypothalamus: 3.6 nTPM
  • amygdala: 3.2 nTPM
  • basal ganglia: 3.2 nTPM
  • cerebral cortex: 3 nTPM
  • white matter: 2.1 nTPM
  • medulla oblongata: 1.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.13
gnomAD pLI
0
gnomAD missense Z
-1.17
DepMap mean gene effect
0.07
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NPFFR2 as an antibody target. Whether an autoantibody or antibody against NPFFR2 could matter depends on whether native NPFFR2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NPFFR2 is annotated at the cell surface, where native NPFFR2 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label NPFFR2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NPFFR2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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