Seroatlas · Human Serome Atlas

NPFFR1

Neuropeptide FF receptor 1

Also known as: GPR147, NPFF1_HUMAN, NPFF1R1, OT7T022

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9GZQ6
Gene: NPFFR1
Ensembl: ENSG00000148734
Chromosome: 10
Canonical length: 430 aa
Protein class: G-protein coupled receptors, Predicted membrane proteins

OverviewNCBI Gene

Enables neuropeptide receptor activity. Involved in neuropeptide signaling pathway. Located in cilium. Is active in plasma membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

430 residues, UniProt reviewed canonical sequence.

>Q9GZQ6|NPFFR1
     1  MEGEPSQPPN SSWPLSQNGT NTEATPATNL TFSSYYQHTS PVAAMFIVAY ALIFLLCMVG
    61  NTLVCFIVLK NRHMHTVTNM FILNLAVSDL LVGIFCMPTT LVDNLITGWP FDNATCKMSG
   121  LVQGMSVSAS VFTLVAIAVE RFRCIVHPFR EKLTLRKALV TIAVIWALAL LIMCPSAVTL
   181  TVTREEHHFM VDARNRSYPL YSCWEAWPEK GMRRVYTTVL FSHIYLAPLA LIVVMYARIA
   241  RKLCQAPGPA PGGEEAADPR ASRRRARVVH MLVMVALFFT LSWLPLWALL LLIDYGQLSA
   301  PQLHLVTVYA FPFAHWLAFF NSSANPIIYG YFNENFRRGF QAAFRARLCP RPSGSHKEAY
   361  SERPGGLLHR RVFVVVRPSD SGLPSESGPS SGAPRPGRLP LRNGRVAHHG LPREGPGCSH
   421  LPLTIPAWDI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NPFFR1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 7
Mean surface accessibility (rSASA): 0.41
Highest tissue expression: 5.5 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 5.5 nTPM
retina: 2.8 nTPM
hypothalamus: 1.8 nTPM
basal ganglia: 1.7 nTPM
spinal cord: 1.7 nTPM
cerebral cortex: 1 nTPM

Single-cell type

bergmann glia: 55 nCPM
retinal pigment epithelial cells: 30 nCPM
müller glia: 22 nCPM
astrocytes: 21 nCPM
other brain neurons: 10 nCPM
brain excitatory neurons: 10 nCPM

Immune cell

total PBMC: 0.7 nTPM
basophil: 0.5 nTPM
classical monocyte: 0.4 nTPM
neutrophil: 0.3 nTPM
myeloid DC: 0.2 nTPM
NK-cell: 0.2 nTPM

Brain region

cerebellum: 21 nTPM
thalamus: 8.2 nTPM
hypothalamus: 7.4 nTPM
midbrain: 7.4 nTPM
white matter: 7.4 nTPM
pons: 7.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.92
gnomAD pLI: 0
gnomAD missense Z: 1.48
DepMap mean gene effect: -0.03
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

G protein-coupled receptor, rhodopsin-like
Neuropeptide FF receptor family
GPCR, rhodopsin-like, 7TM
7 transmembrane receptor (rhodopsin family)
Neuropeptide FF receptor, type 1

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NPFFR1 as an antibody target. Whether an autoantibody or antibody against NPFFR1 could matter depends on whether native NPFFR1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NPFFR1 is annotated at the cell surface, where native NPFFR1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label NPFFR1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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