Seroatlas · Human Serome Atlas

NPFF

Pro-FMRFamide-related neuropeptide FF

Also known as: FMRFAL, NPFF_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O15130
Gene: NPFF
Ensembl: ENSG00000139574
Chromosome: 12
Canonical length: 113 aa
Protein class: Predicted secreted proteins
Secretome location: Secreted in male reproductive system

OverviewNCBI Gene

This gene encodes a member of the FMRFamide related peptide (FARP) family of neuropeptides. The encoded preproprotein is proteolytically processed to generate multiple amidated peptides. These peptides may play a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. Patients with hypertension exhibit decreased expression of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Canonical amino-acid sequenceUniProt

113 residues, UniProt reviewed canonical sequence.

>O15130|NPFF
     1  MDSRQAAALL VLLLLIDGGC AEGPGGQQED QLSAEEDSEP LPPQDAQTSG SLLHYLLQAM
    61  ERPGRSQAFL FQPQRFGRNT QGSWRNEWLS PRAGEGLNSQ FWSLAAPQRF GKK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NPFF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.65
Highest tissue expression: 1,490 nTPM

Expression across tissuesHPA

Tissue

epididymis: 1,490 nTPM
spinal cord: 3.2 nTPM
cerebellum: 0.9 nTPM
hypothalamus: 0.8 nTPM
adrenal gland: 0.7 nTPM
salivary gland: 0.5 nTPM

Single-cell type

epididymal principal cells: 840 nCPM
epididymal clear cells: 14 nCPM
astrocytes: 11 nCPM
epididymal basal cells: 11 nCPM
oligodendrocytes: 4.8 nCPM
bergmann glia: 4.6 nCPM

Immune cell

gdT-cell: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

medulla oblongata: 7.6 nTPM
spinal cord: 6.7 nTPM
midbrain: 6.5 nTPM
hypothalamus: 3 nTPM
cerebral cortex: 1.8 nTPM
thalamus: 1.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.9
gnomAD pLI: 0
gnomAD missense Z: 0.46
DepMap mean gene effect: -0.01
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Pro-FMRFamide-related neuropeptide FF
Neuropeptide FF

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NPFF as an antibody target. Whether an autoantibody or antibody against NPFF could matter depends on whether native NPFF is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NPFF is annotated as secreted, so native NPFF circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label NPFF as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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