Seroatlas · Human Serome Atlas

NPEPPS

Puromycin-sensitive aminopeptidase

Also known as: MP100, PSA, PSA_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P55786
Gene: NPEPPS
Ensembl: ENSG00000141279
Chromosome: 17
Canonical length: 919 aa
Protein class: Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

919 residues, UniProt reviewed canonical sequence.

>P55786|NPEPPS
     1  MWLAAAAPSL ARRLLFLGPP PPPLLLLVFS RSSRRRLHSL GLAAMPEKRP FERLPADVSP
    61  INYSLCLKPD LLDFTFEGKL EAAAQVRQAT NQIVMNCADI DIITASYAPE GDEEIHATGF
   121  NYQNEDEKVT LSFPSTLQTG TGTLKIDFVG ELNDKMKGFY RSKYTTPSGE VRYAAVTQFE
   181  ATDARRAFPC WDEPAIKATF DISLVVPKDR VALSNMNVID RKPYPDDENL VEVKFARTPV
   241  MSTYLVAFVV GEYDFVETRS KDGVCVRVYT PVGKAEQGKF ALEVAAKTLP FYKDYFNVPY
   301  PLPKIDLIAI ADFAAGAMEN WGLVTYRETA LLIDPKNSCS SSRQWVALVV GHELAHQWFG
   361  NLVTMEWWTH LWLNEGFASW IEYLCVDHCF PEYDIWTQFV SADYTRAQEL DALDNSHPIE
   421  VSVGHPSEVD EIFDAISYSK GASVIRMLHD YIGDKDFKKG MNMYLTKFQQ KNAATEDLWE
   481  SLENASGKPI AAVMNTWTKQ MGFPLIYVEA EQVEDDRLLR LSQKKFCAGG SYVGEDCPQW
   541  MVPITISTSE DPNQAKLKIL MDKPEMNVVL KNVKPDQWVK LNLGTVGFYR TQYSSAMLES
   601  LLPGIRDLSL PPVDRLGLQN DLFSLARAGI ISTVEVLKVM EAFVNEPNYT VWSDLSCNLG
   661  ILSTLLSHTD FYEEIQEFVK DVFSPIGERL GWDPKPGEGH LDALLRGLVL GKLGKAGHKA
   721  TLEEARRRFK DHVEGKQILS ADLRSPVYLT VLKHGDGTTL DIMLKLHKQA DMQEEKNRIE
   781  RVLGATLLPD LIQKVLTFAL SEEVRPQDTV SVIGGVAGGS KHGRKAAWKF IKDNWEELYN
   841  RYQGGFLISR LIKLSVEGFA VDKMAGEVKA FFESHPAPSA ERTIQQCCEN ILLNAAWLKR
   901  DAESIHQYLL QRKASPPTV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NPEPPS can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.22
Highest tissue expression: 99 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 99 nTPM
tongue: 67 nTPM
esophagus: 61 nTPM
vagina: 40 nTPM
cervix: 36 nTPM
adrenal gland: 35 nTPM

Single-cell type

esophageal apical cells: 1,613 nCPM
endometrial luminal cells: 556 nCPM
neutrophils: 519 nCPM
endometrial glandular cells: 450 nCPM
urothelial cells: 444 nCPM
esophageal suprabasal cells: 443 nCPM

Immune cell

neutrophil: 25 nTPM
basophil: 13 nTPM
eosinophil: 11 nTPM
plasmacytoid DC: 9 nTPM
myeloid DC: 8.5 nTPM
MAIT T-cell: 8.2 nTPM

Brain region

medulla oblongata: 61 nTPM
cerebral cortex: 60 nTPM
pons: 59 nTPM
midbrain: 57 nTPM
basal ganglia: 55 nTPM
thalamus: 54 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.06
gnomAD pLI: 1
gnomAD missense Z: 3.98
DepMap mean gene effect: -0.45
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NPEPPS as an antibody target. Whether an autoantibody or antibody against NPEPPS could matter depends on whether native NPEPPS is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NPEPPS is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NPEPPS as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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