Seroatlas · Human Serome Atlas

NPEPL1

Probable aminopeptidase NPEPL1

Also known as: bA261P9.2, FLJ11583, PEPL1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NDH3
Gene: NPEPL1
Ensembl: ENSG00000215440
Chromosome: 20
Canonical length: 523 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Predicted to enable peptidase activity. Predicted to be involved in proteolysis. Located in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

523 residues, UniProt reviewed canonical sequence.

>Q8NDH3|NPEPL1
     1  MANVGLQFQA SAGDSDPQSR PLLLLGQLHH LHRVPWSHVR GKLQPRVTEE LWQAALSTLN
    61  PNPTDSCPLY LNYATVAALP CRVSRHNSPS AAHFITRLVR TCLPPGAHRC IVMVCEQPEV
   121  FASACALARA FPLFTHRSGA SRRLEKKTVT VEFFLVGQDN GPVEVSTLQC LANATDGVRL
   181  AARIVDTPCN EMNTDTFLEE INKVGKELGI IPTIIRDEEL KTRGFGGIYG VGKAALHPPA
   241  LAVLSHTPDG ATQTIAWVGK GIVYDTGGLS IKGKTTMPGM KRDCGGAAAV LGAFRAAIKQ
   301  GFKDNLHAVF CLAENSVGPN ATRPDDIHLL YSGKTVEINN TDAEGRLVLA DGVSYACKDL
   361  GADIILDMAT LTGAQGIATG KYHAAVLTNS AEWEAACVKA GRKCGDLVHP LVYCPELHFS
   421  EFTSAVADMK NSVADRDNSP SSCAGLFIAS HIGFDWPGVW VHLDIAAPVH AGERATGFGV
   481  ALLLALFGRA SEDPLLNLVS PLGCEVDVEE GDLGRDSKRR RLV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NPEPL1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.23
Highest tissue expression: 62 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 62 nTPM
cerebellum: 58 nTPM
kidney: 43 nTPM
colon: 42 nTPM
spleen: 42 nTPM
thyroid gland: 42 nTPM

Single-cell type

bergmann glia: 61 nCPM
astrocytes: 52 nCPM
breast lactating cells: 47 nCPM
brain excitatory neurons: 29 nCPM
oligodendrocyte progenitor cells: 28 nCPM
ependymal cells: 28 nCPM

Immune cell

basophil: 24 nTPM
non-classical monocyte: 19 nTPM
classical monocyte: 19 nTPM
neutrophil: 14 nTPM
intermediate monocyte: 13 nTPM
myeloid DC: 11 nTPM

Brain region

medulla oblongata: 25 nTPM
choroid plexus: 25 nTPM
thalamus: 24 nTPM
basal ganglia: 22 nTPM
cerebral cortex: 22 nTPM
white matter: 21 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.07
gnomAD pLI: 0
gnomAD missense Z: 0.51
DepMap mean gene effect: 0.06
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

proteolysis

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Peptidase M17, leucyl aminopeptidase, C-terminal
Peptidase M17, leucine aminopeptidase/peptidase B
Cytosol aminopeptidase family, catalytic domain
Probable aminopeptidase NPEPL1, N-terminal
M17 aminopeptidase N-terminal domain 2

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NPEPL1 as an antibody target. Whether an autoantibody or antibody against NPEPL1 could matter depends on whether native NPEPL1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NPEPL1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NPEPL1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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