Seroatlas · Human Serome Atlas

NPAS4

Neuronal PAS domain-containing protein 4

Also known as: bHLHe79, Le-PAS, NPAS4_HUMAN, NXF, PASD10

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8IUM7
Gene
NPAS4
Ensembl
ENSG00000174576
Chromosome
11
Canonical length
802 aa
Protein class
Predicted intracellular proteins, Transcription factors
Subcellular location
Nucleoplasm,Nuclear membrane,Nuclear bodies,Vesicles

OverviewNCBI Gene

NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (see SIM2; MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events (Ooe et al., 2004 [PubMed 14701734]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

802 residues, UniProt reviewed canonical sequence.

>Q8IUM7|NPAS4
     1  MYRSTKGASK ARRDQINAEI RNLKELLPLA EADKVRLSYL HIMSLACIYT RKGVFFAGGT
    61  PLAGPTGLLS AQELEDIVAA LPGFLLVFTA EGKLLYLSES VSEHLGHSMV DLVAQGDSIY
   121  DIIDPADHLT VRQQLTLPSA LDTDRLFRCR FNTSKSLRRQ SAGNKLVLIR GRFHAHPPGA
   181  YWAGNPVFTA FCAPLEPRPR PGPGPGPGPA SLFLAMFQSR HAKDLALLDI SESVLIYLGF
   241  ERSELLCKSW YGLLHPEDLA HASAQHYRLL AESGDIQAEM VVRLQAKTGG WAWIYCLLYS
   301  EGPEGPITAN NYPISDMEAW SLRQQLNSED TQAAYVLGTP TMLPSFPENI LSQEECSSTN
   361  PLFTAALGAP RSTSFPSAPE LSVVSASEEL PRPSKELDFS YLTFPSGPEP SLQAELSKDL
   421  VCTPPYTPHQ PGGCAFLFSL HEPFQTHLPT PSSTLQEQLT PSTATFSDQL TPSSATFPDP
   481  LTSPLQGQLT ETSVRSYEDQ LTPCTSTFPD QLLPSTATFP EPLGSPAHEQ LTPPSTAFQA
   541  HLDSPSQTFP EQLSPNPTKT YFAQEGCSFL YEKLPPSPSS PGNGDCTLLA LAQLRGPLSV
   601  DVPLVPEGLL TPEASPVKQS FFHYSEKEQN EIDRLIQQIS QLAQGMDRPF SAEAGTGGLE
   661  PLGGLEPLDS NLSLSGAGPP VLSLDLKPWK CQELDFLADP DNMFLEETPV EDIFMDLSTP
   721  DPSEEWGSGD PEAEGPGGAP SPCNNLSPED HSFLEDLATY ETAFETGVSA FPYDGFTDEL
   781  HQLQSQVQDS FHEDGSGGEP TF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NPAS4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.52
Highest tissue expression
20 nTPM

Expression across tissuesHPA

Tissue

  • pituitary gland: 20 nTPM
  • cerebellum: 19 nTPM
  • hypothalamus: 5.2 nTPM
  • basal ganglia: 4.6 nTPM
  • cerebral cortex: 4.2 nTPM
  • hippocampal formation: 4 nTPM

Single-cell type

  • retinal amacrine cells: 22 nCPM
  • lactotrophs: 22 nCPM
  • gonadotrophs: 11 nCPM
  • pancreatic islet cells: 11 nCPM
  • retinal ganglion cells: 7.7 nCPM
  • smooth muscle cells: 6.7 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • cerebellum: 368 nTPM
  • cerebral cortex: 115 nTPM
  • hypothalamus: 18 nTPM
  • white matter: 8.3 nTPM
  • hippocampal formation: 7.1 nTPM
  • basal ganglia: 6.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.32
gnomAD pLI
0.97
gnomAD missense Z
1.52
DepMap mean gene effect
-0.16
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NPAS4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NPAS4 as an antibody target. Whether an autoantibody or antibody against NPAS4 could matter depends on whether native NPAS4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NPAS4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NPAS4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NPAS4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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