NOX3

Protein identityUniProt · HPA

UniProt accession

Q9HBY0

Gene

NOX3

Ensembl

ENSG00000074771

Chromosome

6

Canonical length

568 aa

Protein class

Predicted membrane proteins, Transporters

Subcellular location

Vesicles

OverviewNCBI Gene

This gene encodes a member of the NOX family of NADPH oxidases. These enzymes have the capacity to generate superoxide and other reactive oxygen species (ROS) and transport electrons across the plasma membrane. The ROS generated by family members have been implicated in numerous biological functions including host defense, posttranlational processing of proteins, cellular signaling, regulation of gene expression, and cell differentiation. The protein encoded by this gene is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity.[provided by RefSeq, May 2009]

Canonical amino-acid sequenceUniProt

568 residues, UniProt reviewed canonical sequence.

>Q9HBY0|NOX3
     1  MMGCWILNEG LSTILVLSWL GINFYLFIDT FYWYEEEESF HYTRVILGST LAWARASALC
    61  LNFNCMLILI PVSRNLISFI RGTSICCRGP WRRQLDKNLR FHKLVAYGIA VNATIHIVAH
   121  FFNLERYHWS QSEEAQGLLA ALSKLGNTPN ESYLNPVRTF PTNTTTELLR TIAGVTGLVI
   181  SLALVLIMTS STEFIRQASY ELFWYTHHVF IVFFLSLAIH GTGRIVRGQT QDSLSLHNIT
   241  FCRDRYAEWQ TVAQCPVPQF SGKEPSAWKW ILGPVVLYAC ERIIRFWRFQ QEVVITKVVS
   301  HPSGVLELHM KKRGFKMAPG QYILVQCPAI SSLEWHPFTL TSAPQEDFFS VHIRAAGDWT
   361  AALLEAFGAE GQALQEPWSL PRLAVDGPFG TALTDVFHYP VCVCVAAGIG VTPFAALLKS
   421  IWYKCSEAQT PLKLSKVYFY WICRDARAFE WFADLLLSLE TRMSEQGKTH FLSYHIFLTG
   481  WDENQALHIA LHWDENTDVI TGLKQKTFYG RPNWNNEFKQ IAYNHPSSSI GVFFCGPKAL
   541  SRTLQKMCHL YSSADPRGVH FYYNKESF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NOX3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

6

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

0.1 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.05

gnomAD pLI

0

gnomAD missense Z

-0.23

DepMap mean gene effect

0.11

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• defense response
• detection of gravity
• otolith development
• superoxide anion generation
• temperature homeostasis

Molecular functions

• heme binding
• metal ion binding
• NAD(P)H oxidase H2O2-forming activity
• superoxide-generating NAD(P)H oxidase activity
• superoxide-generating NADPH oxidase activity

Cellular components

• cytoplasm
• extracellular exosome
• NADPH oxidase complex
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Cytochrome b245, heavy chain
• FAD-binding 8
• Ferric reductase, NAD binding domain
• Ferric reductase transmembrane component-like domain
• FAD-binding domain, ferredoxin reductase-type
• Riboflavin synthase-like beta-barrel
• Ferredoxin-NADP reductase (FNR), nucleotide-binding domain
• Respiratory burst oxidase/Ferric reductase
• Ferric reductase like transmembrane component
• FAD-binding domain
• Ferric reductase NAD binding domain

KeywordsUniProt

• Cell membrane
• Glycoprotein
• Heme
• Iron
• Membrane
• Metal-binding
• Oxidoreductase
• Transmembrane
• Transmembrane helix