Seroatlas · Human Serome Atlas

NOTO

Homeobox protein notochord

Also known as: NOTO_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: A8MTQ0
Gene: NOTO
Ensembl: ENSG00000214513
Chromosome: 2
Canonical length: 251 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in brain development; neuron differentiation; and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including embryonic organ development; motile cilium assembly; and regulation of cilium assembly. Predicted to be located in chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

251 residues, UniProt reviewed canonical sequence.

>A8MTQ0|NOTO
     1  MPSPRPRGSP PPAPSGSRVR PPRSGRSPAP RSPTGPNTPR APGRFESPFS VEAILARPDP
    61  CAPAASQPSG SACVHPAFWT AASLCATGGL PWACPTSWLP AYLSVGFYPV PGPRVAPVCG
   121  LLGFGVTGLE LAHCSGLWAF PDWAPTEDLQ DTERQQKRVR TMFNLEQLEE LEKVFAKQHN
   181  LVGKKRAQLA ARLKLTENQV RVWFQNRRVK YQKQQKLRAA VTSAEAASLD EPSSSSIASI
   241  QSDDAESGVD G

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NOTO can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.64
Highest tissue expression: 0.4 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 0.4 nTPM
skin: 0.2 nTPM
adrenal gland: 0.1 nTPM
appendix: 0.1 nTPM
duodenum: 0.1 nTPM
parathyroid gland: 0.1 nTPM

Single-cell type

epididymal basal cells: 1.4 nCPM
syncytiotrophoblasts: 1.2 nCPM
gastric chief cells: 1.1 nCPM
undifferentiated spermatogonia: 1.1 nCPM
late spermatids: 0.5 nCPM
early spermatids: 0.4 nCPM

Immune cell

basophil: 0.4 nTPM
neutrophil: 0.3 nTPM
NK-cell: 0.2 nTPM
classical monocyte: 0.1 nTPM
MAIT T-cell: 0.1 nTPM
memory CD4 T-cell: 0.1 nTPM

Brain region

cerebellum: 1.9 nTPM
medulla oblongata: 1.7 nTPM
pons: 1.6 nTPM
midbrain: 1.5 nTPM
cerebral cortex: 1.4 nTPM
amygdala: 1.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.33
gnomAD pLI: 0
gnomAD missense Z: 0.24
DepMap mean gene effect: -0.17
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NOTO as an antibody target. Whether an autoantibody or antibody against NOTO could matter depends on whether native NOTO is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NOTO is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NOTO as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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