Seroatlas · Human Serome Atlas

NOL4

Nucleolar protein 4

Also known as: CT125, HRIHFB2255, NOL4_HUMAN, NOLP

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O94818
Gene: NOL4
Ensembl: ENSG00000101746
Chromosome: 18
Canonical length: 638 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Predicted to enable RNA binding activity. Predicted to be located in nucleolus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

638 residues, UniProt reviewed canonical sequence.

>O94818|NOL4
     1  MESERDMYRQ FQDWCLRTYG DSGKTKTVTR KKYERIVQLL NGSESSSTDN AKFKFWVKSK
    61  GFQLGQPDEV RGGGGGAKQV LYVPVKTTDG VGVDEKLSLR RVAVVEDFFD IIYSMHVETG
   121  PNGEQIRKHA GQKRTYKAIS ESYAFLPREA VTRFLMSCSE CQKRMHLNPD GTDHKDNGKP
   181  PTLVTSMIDY NMPITMAYMK HMKLQLLNSQ QDEDESSIES DEFDMSDSTR MSAVNSDLSS
   241  NLEERMQSPQ NLHGQQDDDS AAESFNGNET LGHSSIASGG THSREMGDSN SDGKTGLEQD
   301  EQPLNLSDSP LSAQLTSEYR IDDHNSNGKN KYKNLLISDL KMEREARENG SKSPAHSYSS
   361  YDSGKNESVD RGAEDLSLNR GDEDEDDHED HDDSEKVNET DGVEAERLKA FNMFVRLFVD
   421  ENLDRMVPIS KQPKEKIQAI IDSCRRQFPE YQERARKRIR TYLKSCRRMK RSGFEMSRPI
   481  PSHLTSAVAE SILASACESE SRNAAKRMRL ERQQDESAPA DKQCKPEATQ ATYSTSAVPG
   541  SQDVLYINGN GTYSYHSYRG LGGGLLNLND ASSSGPTDLS MKRQLATSSG SSSSSNSRPQ
   601  LSPTEINAVR QLVAGYRESA AFLLRSADEL ENLILQQN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NOL4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.54
Highest tissue expression: 12 nTPM

Expression across tissuesHPA

Tissue

testis: 12 nTPM
cerebral cortex: 8.6 nTPM
cerebellum: 8.5 nTPM
basal ganglia: 7.1 nTPM
pituitary gland: 6.3 nTPM
hypothalamus: 6 nTPM

Single-cell type

corticotrophs: 1,001 nCPM
lactotrophs: 568 nCPM
somatotrophs: 523 nCPM
thyrotrophs: 493 nCPM
oligodendrocyte progenitor cells: 455 nCPM
pancreatic islet cells: 397 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

hypothalamus: 33 nTPM
cerebral cortex: 25 nTPM
cerebellum: 24 nTPM
basal ganglia: 23 nTPM
hippocampal formation: 18 nTPM
amygdala: 18 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.22
gnomAD pLI: 1
gnomAD missense Z: 2.27
DepMap mean gene effect: -0.01
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

RNA binding

Cellular components

nucleolus

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Nucleus

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NOL4 as an antibody target. Whether an autoantibody or antibody against NOL4 could matter depends on whether native NOL4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NOL4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NOL4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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