Seroatlas · Human Serome Atlas

NIT2

Omega-amidase NIT2

Also known as: NIT2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NQR4
Gene: NIT2
Ensembl: ENSG00000114021
Chromosome: 3
Canonical length: 276 aa
Protein class: Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins
Subcellular location: Centrosome,Cytosol
Quaternary structure: Homodimer

OverviewNCBI Gene

Enables omega-amidase activity. Involved in asparagine metabolic process; glutamine metabolic process; and oxaloacetate metabolic process. Located in centrosome and cytosol. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

276 residues, UniProt reviewed canonical sequence.

>Q9NQR4|NIT2
     1  MTSFRLALIQ LQISSIKSDN VTRACSFIRE AATQGAKIVS LPECFNSPYG AKYFPEYAEK
    61  IPGESTQKLS EVAKECSIYL IGGSIPEEDA GKLYNTCAVF GPDGTLLAKY RKIHLFDIDV
   121  PGKITFQESK TLSPGDSFST FDTPYCRVGL GICYDMRFAE LAQIYAQRGC QLLVYPGAFN
   181  LTTGPAHWEL LQRSRAVDNQ VYVATASPAR DDKASYVAWG HSTVVNPWGE VLAKAGTEEA
   241  IVYSDIDLKK LAEIRQQIPV FRQKRSDLYA VEMKKP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NIT2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.22
Highest tissue expression: 25 nTPM

Expression across tissuesHPA

Tissue

liver: 25 nTPM
kidney: 17 nTPM
skeletal muscle: 8.7 nTPM
tongue: 7.1 nTPM
breast: 6.4 nTPM
thyroid gland: 5.5 nTPM

Single-cell type

hepatocytes: 220 nCPM
gastric progenitor cells: 147 nCPM
esophageal suprabasal cells: 132 nCPM
esophageal basal cells: 125 nCPM
decidual stromal cells: 117 nCPM
cytotrophoblasts: 112 nCPM

Immune cell

myeloid DC: 2 nTPM
memory B-cell: 1.5 nTPM
naive B-cell: 1.5 nTPM
MAIT T-cell: 1.4 nTPM
naive CD4 T-cell: 1.2 nTPM
T-reg: 1.2 nTPM

Brain region

basal ganglia: 9.2 nTPM
cerebral cortex: 8.1 nTPM
choroid plexus: 7.9 nTPM
cerebellum: 7 nTPM
hypothalamus: 7 nTPM
white matter: 6.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.77
gnomAD pLI: 0
gnomAD missense Z: 0.4
DepMap mean gene effect: -0.14
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

2-oxoglutaramate amidase activity
omega-amidase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NIT2 as an antibody target. Whether an autoantibody or antibody against NIT2 could matter depends on whether native NIT2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NIT2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NIT2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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