Seroatlas · Human Serome Atlas

NFE2

Transcription factor NF-E2 45 kDa subunit

Also known as: NF-E2, NFE2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q16621
Gene: NFE2
Ensembl: ENSG00000123405
Chromosome: 12
Canonical length: 373 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm,Actin filaments
Quaternary structure: Homodimer

OverviewNCBI Gene

Enables WW domain binding activity; identical protein binding activity; and sequence-specific double-stranded DNA binding activity. Contributes to cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including labyrinthine layer blood vessel development; negative regulation of bone mineralization; and positive regulation of peptidyl-lysine acetylation. Part of RNA polymerase II transcription regulator complex and protein-DNA complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

373 residues, UniProt reviewed canonical sequence.

>Q16621|NFE2
     1  MSPCPPQQSR NRVIQLSTSE LGEMELTWQE IMSITELQGL NAPSEPSFEP QAPAPYLGPP
    61  PPTTYCPCSI HPDSGFPLPP PPYELPASTS HVPDPPYSYG NMAIPVSKPL SLSGLLSEPL
   121  QDPLALLDIG LPAGPPKPQE DPESDSGLSL NYSDAESLEL EGTEAGRRRS EYVEMYPVEY
   181  PYSLMPNSLA HSNYTLPAAE TPLALEPSSG PVRAKPTARG EAGSRDERRA LAMKIPFPTD
   241  KIVNLPVDDF NELLARYPLT ESQLALVRDI RRRGKNKVAA QNCRKRKLET IVQLERELER
   301  LTNERERLLR ARGEADRTLE VMRQQLTELY RDIFQHLRDE SGNSYSPEEY ALQQAADGTI
   361  FLVPRGTKME ATD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NFE2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.57
Highest tissue expression: 106 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 106 nTPM
spleen: 46 nTPM
lung: 13 nTPM
appendix: 5.9 nTPM
choroid plexus: 5.7 nTPM
skin: 4.6 nTPM

Single-cell type

platelets: 652 nCPM
neutrophils: 301 nCPM
megakaryocytes: 210 nCPM
erythrocytes: 98 nCPM
monocyte progenitors: 84 nCPM
neutrophil progenitors: 81 nCPM

Immune cell

basophil: 1,493 nTPM
neutrophil: 814 nTPM
classical monocyte: 242 nTPM
eosinophil: 233 nTPM
total PBMC: 160 nTPM
myeloid DC: 157 nTPM

Brain region

choroid plexus: 2.6 nTPM
cerebral cortex: 1.7 nTPM
pons: 1 nTPM
basal ganglia: 0.9 nTPM
medulla oblongata: 0.9 nTPM
midbrain: 0.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.52
gnomAD pLI: 0.67
gnomAD missense Z: 1.22
DepMap mean gene effect: -0.08
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NFE2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NFE2 as an antibody target. Whether an autoantibody or antibody against NFE2 could matter depends on whether native NFE2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NFE2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NFE2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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