Seroatlas · Human Serome Atlas

NEIL2

Endonuclease 8-like 2

Also known as: FLJ31644, MGC2832, MGC4505, NEH2, NEIL2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q969S2
Gene: NEIL2
Ensembl: ENSG00000154328
Chromosome: 8
Canonical length: 332 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Vesicles

OverviewNCBI Gene

This gene encodes a member of the Fpg/Nei family of DNA glycosylases. These glycosylases initiate the first step in base excision repair by cleaving oxidatively damaged bases and introducing a DNA strand break via their abasic site lyase activity. This enzyme is primarily associated with DNA repair during transcription and acts prefentially on cytosine-derived lesions, particularly 5-hydroxyuracil and 5-hydroxycytosine. It contains an N-terminal catalytic domain, a hinge region, and a C-terminal DNA-binding domain with helix-two-turn-helix and zinc finger motifs. This enzyme interacts with the X-ray cross complementing factor 1 scaffold protein as part of a multi-protein DNA repair complex. A pseudogene of this gene has been identified. [provided by RefSeq, Mar 2017]

Canonical amino-acid sequenceUniProt

332 residues, UniProt reviewed canonical sequence.

>Q969S2|NEIL2
     1  MPEGPLVRKF HHLVSPFVGQ QVVKTGGSSK KLQPASLQSL WLQDTQVHGK KLFLRFDLDE
    61  EMGPPGSSPT PEPPQKEVQK EGAADPKQVG EPSGQKTLDG SSRSAELVPQ GEDDSEYLER
   121  DAPAGDAGRW LRVSFGLFGS VWVNDFSRAK KANKRGDWRD PSPRLVLHFG GGGFLAFYNC
   181  QLSWSSSPVV TPTCDILSEK FHRGQALEAL GQAQPVCYTL LDQRYFSGLG NIIKNEALYR
   241  AGIHPLSLGS VLSASRREVL VDHVVEFSTA WLQGKFQGRP QHTQVYQKEQ CPAGHQVMKE
   301  AFGPEDGLQR LTWWCPQCQP QLSEEPEQCQ FS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NEIL2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.37
Highest tissue expression: 26 nTPM

Expression across tissuesHPA

Tissue

adrenal gland: 26 nTPM
hypothalamus: 25 nTPM
testis: 25 nTPM
midbrain: 25 nTPM
amygdala: 24 nTPM
basal ganglia: 23 nTPM

Single-cell type

other brain neurons: 18 nCPM
brain excitatory neurons: 11 nCPM
brain inhibitory neurons: 11 nCPM
oligodendrocyte progenitor cells: 9 nCPM
choroid plexus epithelial cells: 7.9 nCPM
microglia: 7.8 nCPM

Immune cell

memory B-cell: 22 nTPM
naive CD4 T-cell: 19 nTPM
MAIT T-cell: 18 nTPM
naive B-cell: 17 nTPM
naive CD8 T-cell: 17 nTPM
memory CD8 T-cell: 15 nTPM

Brain region

pons: 51 nTPM
hypothalamus: 48 nTPM
midbrain: 46 nTPM
spinal cord: 40 nTPM
medulla oblongata: 40 nTPM
thalamus: 39 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.74
gnomAD pLI: 0
gnomAD missense Z: -2.09
DepMap mean gene effect: 0.11
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

depyrimidination

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NEIL2 as an antibody target. Whether an autoantibody or antibody against NEIL2 could matter depends on whether native NEIL2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NEIL2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NEIL2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...