Seroatlas · Human Serome Atlas

NDUFB4

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

Also known as: B15, NDUB4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O95168
Gene: NDUFB4
Ensembl: ENSG00000065518
Chromosome: 3
Canonical length: 129 aa
Protein class: Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nucleoplasm,Nuclear membrane,Mitochondria,Principal piece

OverviewNCBI Gene

This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone. [provided by RefSeq, Dec 2009]

Canonical amino-acid sequenceUniProt

129 residues, UniProt reviewed canonical sequence.

>O95168|NDUFB4
     1  MSFPKYKPSS LRTLPETLDP AEYNISPETR RAQAERLAIR AQLKREYLLQ YNDPNRRGLI
    61  ENPALLRWAY ARTINVYPNF RPTPKNSLMG ALCGFGPLIF IYYIIKTERD RKEKLIQEGK
   121  LDRTFHLSY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NDUFB4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.52
Highest tissue expression: 888 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 888 nTPM
heart muscle: 607 nTPM
tongue: 525 nTPM
adrenal gland: 333 nTPM
choroid plexus: 246 nTPM
kidney: 246 nTPM

Single-cell type

late primary spermatocytes: 1,280 nCPM
parietal cells: 1,128 nCPM
early spermatids: 955 nCPM
esophageal apical cells: 940 nCPM
megakaryocytes: 760 nCPM
gastric progenitor cells: 673 nCPM

Immune cell

T-reg: 302 nTPM
plasmacytoid DC: 269 nTPM
non-classical monocyte: 240 nTPM
memory B-cell: 233 nTPM
intermediate monocyte: 231 nTPM
memory CD4 T-cell: 216 nTPM

Brain region

hypothalamus: 112 nTPM
cerebellum: 107 nTPM
choroid plexus: 102 nTPM
white matter: 100 nTPM
cerebral cortex: 98 nTPM
spinal cord: 96 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.43
gnomAD pLI: 0.01
gnomAD missense Z: -0.39
DepMap mean gene effect: -0.54
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

NADH dehydrogenase (ubiquinone) activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

NADH:ubiquinone oxidoreductase, subunit NDUFB4
NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4)

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NDUFB4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NDUFB4 as an antibody target. Whether an autoantibody or antibody against NDUFB4 could matter depends on whether native NDUFB4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NDUFB4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NDUFB4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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