NCLN

Protein identityUniProt · HPA

UniProt accession

Q969V3

Gene

NCLN

Ensembl

ENSG00000125912

Chromosome

19

Canonical length

563 aa

Protein class

Predicted intracellular proteins, Predicted membrane proteins, Transporters

Subcellular location

Nucleoplasm,Lipid droplets

OverviewNCBI Gene

Enables ribosome binding activity. Involved in several processes, including multi-pass transmembrane protein insertion into ER membrane; protein stabilization; and regulation of protein complex stability. Located in endoplasmic reticulum membrane. Part of multi-pass translocon complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

563 residues, UniProt reviewed canonical sequence.

>Q969V3|NCLN
     1  MLEEAGEVLE NMLKASCLPL GFIVFLPAVL LLVAPPLPAA DAAHEFTVYR MQQYDLQGQP
    61  YGTRNAVLNT EARTMAAEVL SRRCVLMRLL DFSYEQYQKA LRQSAGAVVI ILPRAMAAVP
   121  QDVVRQFMEI EPEMLAMETA VPVYFAVEDE ALLSIYKQTQ AASASQGSAS AAEVLLRTAT
   181  ANGFQMVTSG VQSKAVSDWL IASVEGRLTG LGGEDLPTIV IVAHYDAFGV APWLSLGADS
   241  NGSGVSVLLE LARLFSRLYT YKRTHAAYNL LFFASGGGKF NYQGTKRWLE DNLDHTDSSL
   301  LQDNVAFVLC LDTVGRGSSL HLHVSKPPRE GTLQHAFLRE LETVAAHQFP EVRFSMVHKR
   361  INLAEDVLAW EHERFAIRRL PAFTLSHLES HRDGQRSSIM DVRSRVDSKT LTRNTRIIAE
   421  ALTRVIYNLT EKGTPPDMPV FTEQMQIQQE QLDSVMDWLT NQPRAAQLVD KDSTFLSTLE
   481  HHLSRYLKDV KQHHVKADKR DPEFVFYDQL KQVMNAYRVK PAVFDLLLAV GIAAYLGMAY
   541  VAVQHFSLLY KTVQRLLVKA KTQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NCLN can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

60 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NCLN.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 108 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Hirschsprung disease, susceptibility to, 1

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.4

gnomAD pLI

0.76

gnomAD missense Z

1.66

DepMap mean gene effect

-0.05

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• determination of left/right asymmetry in lateral mesoderm
• multi-pass transmembrane protein insertion into ER membrane
• negative regulation of nodal signaling pathway
• protein stabilization
• regulation of protein complex stability
• regulation of protein-containing complex assembly
• regulation of signal transduction

Molecular functions

• ribosome binding

Cellular components

• endoplasmic reticulum membrane
• membrane
• multi-pass translocon complex
• protein-containing complex

Protein domainsUniProt · Pfam · InterPro

• Peptidase M28
• Peptidase family M28
• Nicalin

KeywordsUniProt

• Endoplasmic reticulum
• Glycoprotein
• Membrane
• Signal
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of NCLN in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NOMO1
• NOMO2