NAGPA

Protein identityUniProt · HPA

UniProt accession

Q9UK23

Gene

NAGPA

Ensembl

ENSG00000103174

Chromosome

16

Canonical length

515 aa

Protein class

Disease related genes, Enzymes, Metabolic proteins, Plasma proteins, Potential drug targets, Predicted intracellular proteins, Predicted membrane proteins

Quaternary structure

Homotetramer

OverviewNCBI Gene

Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients. [provided by RefSeq, Oct 2015]

Canonical amino-acid sequenceUniProt

515 residues, UniProt reviewed canonical sequence.

>Q9UK23|NAGPA
     1  MATSTGRWLL LRLALFGFLW EASGGLDSGA SRDDDLLLPY PRARARLPRD CTRVRAGNRE
    61  HESWPPPPAT PGAGGLAVRT FVSHFRDRAV AGHLTRAVEP LRTFSVLEPG GPGGCAARRR
   121  ATVEETARAA DCRVAQNGGF FRMNSGECLG NVVSDERRVS SSGGLQNAQF GIRRDGTLVT
   181  GYLSEEEVLD TENPFVQLLS GVVWLIRNGS IYINESQATE CDETQETGSF SKFVNVISAR
   241  TAIGHDRKGQ LVLFHADGQT EQRGINLWEM AEFLLKQDVV NAINLDGGGS ATFVLNGTLA
   301  SYPSDHCQDN MWRCPRQVST VVCVHEPRCQ PPDCHGHGTC VDGHCQCTGH FWRGPGCDEL
   361  DCGPSNCSQH GLCTETGCRC DAGWTGSNCS EECPLGWHGP GCQRPCKCEH HCPCDPKTGN
   421  CSVSRVKQCL QPPEATLRAG ELSFFTRTAW LALTLALAFL LLISTAANLS LLLSRAERNR
   481  RLHGDYAYHP LQEMNGEPLA AEKEQPGGAH NPFKD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NAGPA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.33

Highest tissue expression

23 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.32

gnomAD pLI

0

gnomAD missense Z

-1.37

DepMap mean gene effect

-0.16

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• carbohydrate metabolic process
• lysosome organization
• protein modification process
• protein targeting to lysosome
• secretion of lysosomal enzymes

Molecular functions

• N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase activity

Cellular components

• Golgi cisterna membrane
• membrane

Protein domainsUniProt · Pfam · InterPro

• EGF-like domain
• Phosphodiester glycosidase
• Phosphodiester glycosidase

KeywordsUniProt

• Disulfide bond
• EGF-like domain
• Glycoprotein
• Golgi apparatus
• Hydrolase
• Membrane
• Signal
• Transmembrane
• Transmembrane helix
• Zymogen

InteractionsUniProt · HPA

Protein binding partners of NAGPA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• AP4M1