Seroatlas · Human Serome Atlas

NACAD

NAC-alpha domain-containing protein 1

Also known as: KIAA0363, NACAD_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O15069
Gene: NACAD
Ensembl: ENSG00000136274
Chromosome: 7
Canonical length: 1562 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Predicted to enable unfolded protein binding activity. Predicted to be involved in protein targeting to membrane. Predicted to be located in nucleus. Predicted to be part of nascent polypeptide-associated complex. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1562 residues, UniProt reviewed canonical sequence.

>O15069|NACAD
     1  MPGEAARAEL LLPEADRPGP RTDLSCDAAA ATTILGGDRR EPCALTPGPS HLALTFLPSK
    61  PGARPQPEGA SWDAGPGGAP SAWADPGEGG PSPMLLPEGL SSQALSTEAP LPATLEPRIV
   121  MGEETCQALL SPRAARTALR DQEGGHASPD PPPELCSQGD LSVPSPPPDP DSFFTPPSTP
   181  TKTTYALLPA CGPHGDARDS EAELRDELLD SPPASPSGSY ITADGDSWAS SPSCSLSLLA
   241  PAEGLDFPSG WGLSPQGSMV DERELHPAGT PEPPSSESSL SADSSSSWGQ EGHFFDLDFL
   301  ANDPMIPAAL LPFQGSLIFQ VEAVEVTPLS PEEEEEEAVA DPDPGGDLAG EGEEDSTSAS
   361  FLQSLSDLSI TEGMDEAFAF RDDTSAASSD SDSASYAEAD DERLYSGEPH AQATLLQDSV
   421  QKTEEESGGG AKGLQAQDGT VSWAVEAAPQ TSDRGAYLSQ RQELISEVTE EGLALGQEST
   481  ATVTPHTLQV APGLQVEVAT RVTPQAGEEE TDSTAGQESA AMAMPQPSQE GISEILGQES
   541  VTAEKLPTPQ EETSLTLCPD SPQNLKEEGG LDLPSGRKPV AAATIVPRQA KEDLTLPQDS
   601  AMTPPLPLQD TDLSSAPKPV AAATIVSQQA EEGLTLPQDS VMTPPLPLQD TELSSAPKPV
   661  AAATLVSQQA EEGLTLPQDS AMTPPLPLQD TDLSSAPKPV AAATLVSQQA EEGLTLPQDS
   721  AMTPPLPLQD TDLSSAPKPV AAATLVSQQA EEGLTLPQDS AMTPPLPLQD TDLSSAPKPV
   781  AAATIVSQQA EEGLTLPQDS AMTPPLPLQD TDLSSAPKPV AAATIVSQQA EEGLTLPQDS
   841  AMTPPLPLQD TDLSSAPKPV AAATPVSQQA EEGLTLPQDS AMTPPLPLQD TDLSSAPKPV
   901  AAATPVSQQA EEGLTLPQDS AMTAPLPLQD TGPTSGPEPL AVATPQTLQA EAGCAPGTEP
   961  VATMAQQEVG EALGPRPAPE EKNAALPTVP EPAALDQVQQ DDPQPAAEAG TPWAAQEDAD
  1021  STLGMEALSL PEPASGAGEE IAEALSRPGR EACLEARAHT GDGAKPDSPQ KETLEVENQQ
  1081  EGGLKPLAQE HGPRSALGGA REVPDAPPAA CPEVSQARLL SPAREERGLS GKSTPEPTLP
  1141  SAVATEASLD SCPESSVGAV SSLDRGCPDA PAPTSAPTSQ QPEPVLGLGS VEQPHEVPSV
  1201  LGTPLLQPPE NLAKGQPSTP VDRPLGPDPS APGTLAGAAL PPLEPPAPCL CQDPQEDSVE
  1261  DEEPPGSLGL PPPQAGVQPA AAAVSGTTQP LGTGPRVSLS PHSPLLSPKV ASMDAKDLAL
  1321  QILPPCQVPP PSGPQSPAGP QGLSAPEQQE DEDSLEEDSP RALGSGQHSD SHGESSAELD
  1381  EQDILAPQTV QCPAQAPAGG SEETIAKAKQ SRSEKKARKA MSKLGLRQIQ GVTRITIQKS
  1441  KNILFVIAKP DVFKSPASDT YVVFGEAKIE DLSQQVHKAA AEKFKVPSEP SALVPESAPR
  1501  PRVRLECKEE EEEEEEEVDE AGLELRDIEL VMAQANVSRA KAVRALRDNH SDIVNAIMEL
  1561  TM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NACAD can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.66
Highest tissue expression: 63 nTPM

Expression across tissuesHPA

Tissue

spinal cord: 63 nTPM
midbrain: 38 nTPM
cerebellum: 32 nTPM
hippocampal formation: 31 nTPM
hypothalamus: 29 nTPM
cerebral cortex: 28 nTPM

Single-cell type

oligodendrocytes: 40 nCPM
other brain neurons: 18 nCPM
gonadotrophs: 15 nCPM
retinal horizontal cells: 14 nCPM
lactotrophs: 12 nCPM
peritubular myoid cells: 11 nCPM

Immune cell

gdT-cell: 0.2 nTPM
non-classical monocyte: 0.2 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
intermediate monocyte: 0 nTPM

Brain region

white matter: 200 nTPM
pons: 157 nTPM
medulla oblongata: 157 nTPM
thalamus: 151 nTPM
cerebellum: 146 nTPM
cerebral cortex: 136 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.41
gnomAD pLI: 0.16
gnomAD missense Z: 2.37
DepMap mean gene effect: 0.03
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

unfolded protein binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

Nascent polypeptide-associated complex NAC domain
Nascent polypeptide-associated complex subunit alpha-like
NAC A/B domain superfamily
Nascent polypeptide-associated complex subunit alpha-like, UBA domain
NAC domain
HYPK UBA domain
NAC-alpha domain-containing protein 1, UBA-like domain

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NACAD as an antibody target. Whether an autoantibody or antibody against NACAD could matter depends on whether native NACAD is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NACAD is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NACAD as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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