MYLK4
Myosin light chain kinase family member 4
Also known as: MYLK4_HUMAN, SgK085
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q86YV6
- Gene
- MYLK4
- Ensembl
- ENSG00000145949
- Chromosome
- 6
- Canonical length
- 388 aa
- Protein class
- Enzymes, Metabolic proteins, Predicted membrane proteins
- Subcellular location
- Nucleoli,Golgi apparatus,Vesicles
OverviewNCBI Gene
Predicted to enable myosin light chain kinase activity. Predicted to be involved in signal transduction. Predicted to be located in membrane. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
388 residues, UniProt reviewed canonical sequence.
>Q86YV6|MYLK4
1 MLKVKRLEEF NTCYNSNQLE KMAFFQCREE VEKVKCFLEK NSGDQDSRSG HNEAKEVWSN
61 ADLTERMPVK SKRTSALAVD IPAPPAPFDH RIVTAKQGAV NSFYTVSKTE ILGGGRFGQV
121 HKCEETATGL KLAAKIIKTR GMKDKEEVKN EISVMNQLDH ANLIQLYDAF ESKNDIVLVM
181 EYVDGGELFD RIIDESYNLT ELDTILFMKQ ICEGIRHMHQ MYILHLDLKP ENILCVNRDA
241 KQIKIIDFGL ARRYKPREKL KVNFGTPEFL APEVVNYDFV SFPTDMWSVG VIAYMLLSGL
301 SPFLGDNDAE TLNNILACRW DLEDEEFQDI SEEAKEFISK LLIKEKSWRI SASEALKHPW
361 LSDHKLHSRL NAQKKKNRGS DAQDFVTKLocalizationUniProt · AlphaFold · HPA
Whether an antibody against MYLK4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.33
- Highest tissue expression
- 67 nTPM
Expression across tissuesHPA
Tissue
- tongue: 67 nTPM
- skeletal muscle: 27 nTPM
- kidney: 2.7 nTPM
- salivary gland: 2.3 nTPM
- heart muscle: 1.4 nTPM
- pituitary gland: 1.3 nTPM
Single-cell type
- myonuclei: 165 nCPM
- cardiomyocytes: 79 nCPM
- epicardial cells: 36 nCPM
- hematopoietic stem cells: 36 nCPM
- gonadotrophs: 33 nCPM
- retinal amacrine cells: 26 nCPM
Immune cell
- memory CD8 T-cell: 0.1 nTPM
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- eosinophil: 0 nTPM
- gdT-cell: 0 nTPM
- intermediate monocyte: 0 nTPM
Brain region
- cerebral cortex: 2.3 nTPM
- cerebellum: 1.9 nTPM
- hippocampal formation: 1.9 nTPM
- white matter: 1.9 nTPM
- basal ganglia: 1.8 nTPM
- choroid plexus: 1.8 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.37
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.78
- DepMap mean gene effect
- 0.13
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of MYLK4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads MYLK4 as an antibody target. Whether an autoantibody or antibody against MYLK4 could matter depends on whether native MYLK4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
MYLK4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label MYLK4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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