Seroatlas · Human Serome Atlas

MYLK3

Myosin light chain kinase 3

Also known as: caMLCK, MLCK, MYLK3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q32MK0
Gene
MYLK3
Ensembl
ENSG00000140795
Chromosome
16
Canonical length
819 aa
Protein class
Enzymes, Metabolic proteins, Predicted membrane proteins
Subcellular location
Nucleoplasm,Vesicles,Cytosol

OverviewNCBI Gene

Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).[supplied by OMIM, Jul 2008]

Canonical amino-acid sequenceUniProt

819 residues, UniProt reviewed canonical sequence.

>Q32MK0|MYLK3
     1  MSGTSKESLG HGGLPGLGKT CLTTMDTKLN MLNEKVDQLL HFQEDVTEKL QSMCRDMGHL
    61  ERGLHRLEAS RAPGPGGADG VPHIDTQAGW PEVLELVRAM QQDAAQHGAR LEALFRMVAA
   121  VDRAIALVGA TFQKSKVADF LMQGRVPWRR GSPGDSPEEN KERVEEEGGK PKHVLSTSGV
   181  QSDAREPGEE SQKADVLEGT AERLPPIRAS GLGADPAQAV VSPGQGDGVP GPAQAFPGHL
   241  PLPTKVEAKA PETPSENLRT GLELAPAPGR VNVVSPSLEV APGAGQGASS SRPDPEPLEE
   301  GTRLTPGPGP QCPGPPGLPA QARATHSGGE TPPRISIHIQ EMDTPGEMLM TGRGSLGPTL
   361  TTEAPAAAQP GKQGPPGTGR CLQAPGTEPG EQTPEGAREL SPLQESSSPG GVKAEEEQRA
   421  GAEPGTRPSL ARSDDNDHEV GALGLQQGKS PGAGNPEPEQ DCAARAPVRA EAVRRMPPGA
   481  EAGSVVLDDS PAPPAPFEHR VVSVKETSIS AGYEVCQHEV LGGGRFGQVH RCTEKSTGLP
   541  LAAKIIKVKS AKDREDVKNE INIMNQLSHV NLIQLYDAFE SKHSCTLVME YVDGGELFDR
   601  ITDEKYHLTE LDVVLFTRQI CEGVHYLHQH YILHLDLKPE NILCVNQTGH QIKIIDFGLA
   661  RRYKPREKLK VNFGTPEFLA PEVVNYEFVS FPTDMWSVGV ITYMLLSGLS PFLGETDAET
   721  MNFIVNCSWD FDADTFEGLS EEAKDFVSRL LVKEKSCRMS ATQCLKHEWL NNLPAKASRS
   781  KTRLKSQLLL QKYIAQRKWK KHFYVVTAAN RLRKFPTSP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MYLK3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.5
Highest tissue expression
97 nTPM

Expression across tissuesHPA

Tissue

  • heart muscle: 97 nTPM
  • tongue: 68 nTPM
  • skeletal muscle: 43 nTPM
  • testis: 5.5 nTPM
  • esophagus: 4 nTPM
  • hippocampal formation: 2.5 nTPM

Single-cell type

  • thymic myoid cells: 526 nCPM
  • cardiomyocytes: 347 nCPM
  • ependymal cells: 281 nCPM
  • myonuclei: 210 nCPM
  • sertoli cells: 112 nCPM
  • endometrial ciliated cells: 98 nCPM

Immune cell

  • basophil: 0.4 nTPM
  • neutrophil: 0.4 nTPM
  • classical monocyte: 0.1 nTPM
  • memory B-cell: 0.1 nTPM
  • naive B-cell: 0.1 nTPM
  • NK-cell: 0.1 nTPM

Brain region

  • midbrain: 28 nTPM
  • medulla oblongata: 24 nTPM
  • pons: 16 nTPM
  • spinal cord: 15 nTPM
  • white matter: 8.1 nTPM
  • hypothalamus: 8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.72
gnomAD pLI
0
gnomAD missense Z
0.5
DepMap mean gene effect
0.02
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MYLK3 as an antibody target. Whether an autoantibody or antibody against MYLK3 could matter depends on whether native MYLK3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MYLK3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MYLK3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MYLK3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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