MSLN

Protein identityUniProt · HPA

UniProt accession

Q13421

Gene

MSLN

Ensembl

ENSG00000102854

Chromosome

16

Canonical length

630 aa

Protein class

Cancer-related genes, Disease related genes, Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Vesicles,Plasma membrane

Secretome location

Intracellular and membrane

OverviewNCBI Gene

This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Canonical amino-acid sequenceUniProt

630 residues, UniProt reviewed canonical sequence.

>Q13421|MSLN
     1  MALPTARPLL GSCGTPALGS LLFLLFSLGW VQPSRTLAGE TGQEAAPLDG VLANPPNISS
    61  LSPRQLLGFP CAEVSGLSTE RVRELAVALA QKNVKLSTEQ LRCLAHRLSE PPEDLDALPL
   121  DLLLFLNPDA FSGPQACTRF FSRITKANVD LLPRGAPERQ RLLPAALACW GVRGSLLSEA
   181  DVRALGGLAC DLPGRFVAES AEVLLPRLVS CPGPLDQDQQ EAARAALQGG GPPYGPPSTW
   241  SVSTMDALRG LLPVLGQPII RSIPQGIVAA WRQRSSRDPS WRQPERTILR PRFRREVEKT
   301  ACPSGKKARE IDESLIFYKK WELEACVDAA LLATQMDRVN AIPFTYEQLD VLKHKLDELY
   361  PQGYPESVIQ HLGYLFLKMS PEDIRKWNVT SLETLKALLE VNKGHEMSPQ APRRPLPQVA
   421  TLIDRFVKGR GQLDKDTLDT LTAFYPGYLC SLSPEELSSV PPSSIWAVRP QDLDTCDPRQ
   481  LDVLYPKARL AFQNMNGSEY FVKIQSFLGG APTEDLKALS QQNVSMDLAT FMKLRTDAVL
   541  PLTVAEVQKL LGPHVEGLKA EERHRPVRDW ILRQRQDDLD TLGLGLQGGI PNGYLVLDLS
   601  MQEALSGTPC LLGPGPVLTV LALLLASTLA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MSLN can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.35

Highest tissue expression

262 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MSLN.

Disease | ImmuneIEDB

Conditions an epitope on MSLN was assayed in.

• pancreatic ductal adenocarcinoma T cell
• pancreatic cancer T cell
• malignant pleural mesothelioma T cell
• pancreas disease T cell
• malignant mesothelioma T cell
• colorectal cancer T cell

ReferencesPubMed · IEDB

Publications for MSLN from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.48

gnomAD pLI

0

gnomAD missense Z

-1.04

DepMap mean gene effect

0.07

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell adhesion
• cell-matrix adhesion

Cellular components

• cell surface
• endoplasmic reticulum lumen
• extracellular region
• Golgi apparatus
• membrane
• plasma membrane
• side of membrane

Protein domainsUniProt · Pfam · InterPro

• Mesothelin
• Stereocilin-related
• Mesothelin

KeywordsUniProt

• Cell adhesion
• Cell membrane
• Cleavage on pair of basic residues
• Disulfide bond
• Glycoprotein
• Golgi apparatus
• GPI-anchor
• Lipoprotein
• Membrane
• Phosphoprotein
• Secreted
• Signal

InteractionsUniProt · HPA

Protein binding partners of MSLN in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MUC16